1. Effective detection of Lily Symptomless Virus using the reverse transcription loop–mediated isothermal amplification method
- Author
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Yanan Du, Wenjing Wu, Dan Yang, Zhao Xiao, Qi Zhang, Zhao Kai, Jieling Liang, Zhao Huanzhen, Yongchun Zhang, Fan Xiaorui, Ziping Zhu, Wanjing Zhang, Cai Youming, Xiaoxia Zhang, and Yan Xu
- Subjects
0106 biological sciences ,0301 basic medicine ,Lily symptomless virus ,genetic structures ,Loop-mediated isothermal amplification ,Plant Science ,Biology ,01 natural sciences ,Virology ,eye diseases ,Reverse transcriptase ,03 medical and health sciences ,030104 developmental biology ,Visual detection ,Plant virus ,Reverse Transcription Loop-mediated Isothermal Amplification ,010606 plant biology & botany - Abstract
Lily Symptomless Virus (LSV) is prevalent in lily, causing severe economic losses in Lily production. Therefore, it is quite necessary to develop an effective method for detecting LSV. This study aimed to develop a visual Reverse Transcription Loop–Mediated Isothermal Amplification (RT-LAMP) method to detect LSV. The optimum condition was 60 min at 65 °C. The primers designed were highly specific to LSV and did not cross-react with other seven Lily viruses. The sensitivity of RT-LAMP assay for LSV was 0.0001 ng/ul. This RT-LAMP method was rapid, simple, highly specific, and sensitive and helped in the reliable visual detection of LSV in Lily plants.
- Published
- 2019
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