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To improve the simulation performance of the RegCM4 model in climate simulations over the Yangtze River Basin (YRB), it is essential to determine the optimal cumulus convection and land surface process schemes from the numerous physical parameterization options within RegCM4. In this study, we selected five cumulus convection schemes (Kuo, Grell, Emanuel, Tiedtke, and Kain–Fritsch) and three land surface process schemes (BATS, CLM3.5, and CLM4.5) to configure 72 mixed schemes. Four years of short-term simulations (1990–1993) with a horizontal resolution of 50 km were conducted using ERA-Interim as the initial and boundary conditions for the 72 schemes. The climate simulation performance of all schemes in the YRB was comprehensively evaluated using a multi-criteria scoring approach. The results indicate that among the selected cumulus convection schemes, the Kain–Fritsch scheme, applied to both ocean and land, demonstrates optimal performance in simulating precipitation over the YRB, with spatial correlation coefficients between simulated and observed annual precipitation around 0.3. Compared to the Community Land Models (CLM3.5 and CLM4.5), BATS exhibits superior capabilities in reproducing the temperature features of the region, with spatial correlation coefficients between simulated and observed values typically exceeding 0.99 and standard deviations within 1.25 °C. Under the optimal KF scheme, the simulated soil moisture in the YRB using CLMs is notably drier, ranging from −7.79 to −8.39 kg/m2, compared to that achieved with BATS. The findings provide a localized reference for the parameterization schemes of RegCM4 in the YRB. [ABSTRACT FROM AUTHOR]