1. The importance of post-thaw subculture for standardizing cellular activity of fresh or cryopreserved mouse embryonic stem cells
- Author
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Dong Woo Ko, Jung Ki Yoon, Jong il Ahn, Myungook Lee, Woo Sub Yang, Ji Yeon Ahn, and Jeong Mook Lim
- Subjects
Apoptosis ,ATP ,Cryopreservation ,Embryonic Stem Cells ,Mouse ,Reactive Oxygen Species ,Animal culture ,SF1-1100 ,Animal biochemistry ,QP501-801 - Abstract
Objective Remarkable difference in cellular activity was found between early and late subpassaged embryonic stem cell (ESCs) lines, which can be created by subtle changes in cell manipulation protocol. This study subsequently examined whether post-thaw subculture of early subpassaged ESC lines could further affect the activity of the ESCs. Methods Fresh (as a control treatment) or cryopreserved F1 hybrid (B6CBAF1) early ESC lines (C57BL/6xCBA) of the 4 (P4) or the 19 passage (P19) were subcultured once, twice or six times under the same condition. The post-thaw survival of the ESCs was monitored after the post-treatment subculture and the ability of cell proliferation, reactive oxygen species (ROS) generation, apoptosis and mitochondrial ATP synthesis was subsequently examined. Results Regardless of the subculture number, P19 ESCs showed better (p
- Published
- 2018
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