Search

Your search keyword '"depth filter"' showing total 2 results
Start Over Descriptor "depth filter" Journal artificial organs
2 results on '"depth filter"'

1. Release of (1→3)-β-D-Glucan from Depth-type Membrane Filters and Their In Vitro Effects on Proinflammatory Cytokine Production.

Author

Ohata, Atsushi, Usami, Makoto, Horiuchi, Takashi, Nagasawa, Koichi, and Kinoshita, Keiko

Subjects
*GLUCANS, *MEMBRANE filters, *CYTOKINES, *CELL culture, *SCANNING electron microscopy
Abstract

To clarify the origin of (1→3)-β-D-glucan in blood products and assess the biological activity of filter extracts, we evaluated (1→3)-β-D-glucan extraction from depth filters used to process blood products and their in vitro effects on proinflammatory cytokine production from macrophages. Cellulose or nylon filters were analyzed for (1→3)-β-D-glucan using the Fungitec G test. To evaluate the biological activity of the filter extracts, Mono Mac 6 cells (a human macrophage cell line) were cultured with filter extracts with or without lipopolysaccharide, and tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) concentrations in the culture media were measured. (1→3)-β-D-Glucan was released from seven cellulose filters but the nylon filter level was undetectable. Proinflammatory cytokine production ranged from 74.3% to 119.0% of the control for TNF-α and 81.2% to 115.9% for IL-1β. TNF-α and IL-1β levels were low without lipopolysaccharide. The data indicate that (1→3)-β-D-glucan in blood products is contaminated with the depth filters and that these filter extracts modulate proinflammatory cytokine production from macrophages. [ABSTRACT FROM AUTHOR]

Published
2003
Full Text
View/download PDF

2. Release of (13)-beta-D-Glucan from Depth-type Membrane Filters and Their In Vitro Effects on Proinflammatory Cytokine Production

Author

Takashi Horiuchi, Keiko Kinoshita, Makoto Usami, Atsushi Ohata, and Koichi Nagasawa

Subjects
beta-Glucans, Lipopolysaccharide, Biomedical Engineering, Medicine (miscellaneous), Bioengineering, Hemodiafiltration, In Vitro Techniques, Biology, Receptors, Tumor Necrosis Factor, Cell Line, Microbiology, Proinflammatory cytokine, Biomaterials, chemistry.chemical_compound, Blood product, Humans, Glucans, Biological activity, General Medicine, In vitro, chemistry, Cell culture, Depth filter, Microscopy, Electron, Scanning, Tumor necrosis factor alpha, Interleukin-1
Abstract

To clarify the origin of (1-->3)-beta-D-glucan in blood products and assess the biological activity of filter extracts, we evaluated (1-->3)-beta-D-glucan extraction from depth filters used to process blood products and their in vitro effects on proinflammatory cytokine production from macrophages. Cellulose or nylon filters were analyzed for (1-->3)-beta-D-glucan using the Fungitec G test. To evaluate the biological activity of the filter extracts, Mono Mac 6 cells (a human macrophage cell line) were cultured with filter extracts with or without lipopolysaccharide, and tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1beta) concentrations in the culture media were measured. (1-->3)-beta-D-Glucan was released from seven cellulose filters but the nylon filter level was undetectable. Proinflammatory cytokine production ranged from 74.3% to 119.0% of the control for TNF-alpha and 81.2% to 115.9% for IL-1beta. TNF-alpha and IL-1beta levels were low without lipopolysaccharide. The data indicate that (1-->3)-beta-D-glucan in blood products is contaminated with the depth filters and that these filter extracts modulate proinflammatory cytokine production from macrophages.

Published
2003

Catalog

Books, media, physical & digital resources
See catalog results