1. First evidence of the P-glycoprotein gene expression and multixenobiotic resistance modulation in earthworm.
- Author
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Bošnjak I, Bielen A, Babić S, Sver L, Popović NT, Strunjak-Perović I, Což-Rakovac R, and Klobučar RS
- Subjects
- ATP Binding Cassette Transporter, Subfamily B, Member 1 antagonists & inhibitors, ATP Binding Cassette Transporter, Subfamily B, Member 1 chemistry, Amino Acid Sequence, Animals, Base Sequence, DNA, Complementary chemistry, Gene Expression, Molecular Sequence Data, Oligochaeta chemistry, Rhodamine 123 analysis, Rhodamines chemistry, ATP Binding Cassette Transporter, Subfamily B, Member 1 genetics, Drug Resistance, Multiple genetics, Oligochaeta genetics, Proteins chemistry, Xenobiotics pharmacology
- Abstract
Multixenobiotic resistance (MXR) is an important mechanism of cellular efflux mediated by ATP binding cassette (ABC) transporters that bind and actively remove toxic substrates from the cell. This study was the first to identify ABC transporter P-glycoprotein (P-gp/ABCB1) as a representative of the MXR phenotype in earthworm (Eisenia fetida). The identified partial cDNA sequence of ABCB1 overlapped with ABCB1 homologues of other organisms from 58.5 % to 72.5 %. We also studied the effect of five modulators (verapamil, cyclosporine A, MK571, probenecid, and orthovanadate) on the earthworm's MXR activity by measuring the accumulation of model substrates rhodamine B and rhodamine 123 in whole body tissue of the adult earthworm. MK571, orthovanadate, and verapamil significantly inhibited MXR activity, and rhodamine 123 turned out to better reflect MXR activity in that species than rhodamine B. Our results show that E. fetida can serve well as a test organism for environmental pollutants that inhibit MXR activity.
- Published
- 2014
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