1. Molecular detection and phylogenetic analysis of megalocytivirus in Brazilian ornamental fish.
- Author
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de Lucca Maganha SR, Cardoso PHM, de Carvalho Balian S, de Almeida-Queiroz SR, Fernandes AM, and de Sousa RLM
- Subjects
- Animals, Aquaculture, Brazil, Capsid Proteins genetics, DNA Virus Infections virology, Fishes classification, Fishes virology, Iridoviridae classification, Iridoviridae isolation & purification, Polymerase Chain Reaction, DNA Virus Infections veterinary, Fish Diseases virology, Iridoviridae genetics, Phylogeny
- Abstract
Megalocytiviruses have a worldwide distribution, causing serious economic loss to the global aquaculture industry. They also present a threat to ornamental fish trade because megalocytiviral infections have unspecified symptoms, making early diagnosis difficult. In this study, 100 ornamental fish from 24 different species were tested by PCR for megalocytivirus, with a 47% positive rate being identified. Phylogenetic reconstruction, based on the major capsid protein (MCP) gene, clustered all Brazilian samples into a single clade, showing identity values ranging from 99% to 100% when compared to each other. This is the first report of megalocytivirus infection in some ornamental fish species in Brazil.
- Published
- 2018
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