1. Molecular characterization of artemisia virus A, a new sobemovirus isolated from Artemisia annua
- Author
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Xavier Simonnet, Carole Balmelli, Paul Gugerli, Sébastien Besse, and Maria-Elena Ramel
- Subjects
Molecular Sequence Data ,Artemisia annua ,Genome, Viral ,Biology ,Plant Roots ,Genome ,Virus ,Sobemovirus ,Plant Viruses ,Open Reading Frames ,Microscopy, Electron, Transmission ,Virology ,Botany ,Cluster Analysis ,RNA Viruses ,Phylogeny ,Plant Diseases ,Phylogenetic tree ,Virion ,Nucleic acid sequence ,food and beverages ,Sequence Analysis, DNA ,General Medicine ,biology.organism_classification ,Plant Leaves ,Open reading frame ,RNA, Viral ,Artemisia ,Switzerland - Abstract
A distinct sobemovirus was isolated from diseased Artemisia annua plants grown in experimental culture plots in Switzerland. Electron microscopy performed on extracts of leaf and root samples of a diseased A. annua plant revealed icosahedral-30 nm viral particles. The complete nucleotide sequence of the viral genome was determined. The single positive-strand RNA of 4138 nt encodes four open reading frames with an organization similar to that described for sobemoviruses. Phylogenetic analysis revealed a close relationship to ryegrass mottle virus. The virus was efficiently acquired by healthy A. annua from contaminated soil samples. "Artemisia virus A" is tentatively proposed as a name for this new candidate member of the unassigned genus Sobemovirus.
- Published
- 2012
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