1. Characterization of vB_ApiM_fHyAci03, a novel lytic bacteriophage that infects clinical Acinetobacter strains
- Author
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Saija Kiljunen, Elsi Pulkkinen, Mikael Skurnik, Joseph Michael Ochieng’ Oduor, Anu Wicklund, HUSLAB, Research Programs Unit, University of Helsinki, HUMI - Human Microbiome Research, Faculty of Medicine, Helsinki One Health (HOH), Mikael Skurnik / Principal Investigator, and Department of Bacteriology and Immunology
- Subjects
Phage therapy ,medicine.medical_treatment ,Genome, Viral ,Genome ,Host Specificity ,Bacteriophage ,03 medical and health sciences ,Annotated Sequence Record ,Virology ,medicine ,Bacteriophages ,Phylogeny ,030304 developmental biology ,Genetics ,0303 health sciences ,Acinetobacter pittii ,biology ,Acinetobacter ,030306 microbiology ,General Medicine ,biology.organism_classification ,3. Good health ,Acinetobacter baumannii ,Lytic cycle ,Myoviridae ,DNA, Viral ,1182 Biochemistry, cell and molecular biology ,3111 Biomedicine ,Acinetobacter nosocomialis - Abstract
We present here the isolation and characterization of Acinetobacter pittii phage vB_ApiM_fHyAci03 (fHyAci03), which belongs to the family Myoviridae. The fHyAci03 genome was found to be 165,975 bp in length and predicted to contain 255 genes. While the whole genome was 92.4% identical to Acinetobacter baumannii phage KARL-1, phylogenetic analysis based on phage long distal tail fiber amino acid sequences assigned fHyAci03 and KARL-1 to different subclusters, reflecting their different host species. Together with phylogenetic analysis, genome comparisons indicated that fHyAci03 is a novel member of the subfamily Tevenvirinae. Host range experiments revealed that fHyAci03 could infect two clinical strains of Acinetobacter nosocomialis and six clinical strains of A. pittii. Thus, fHyAci03 is a novel lytic phage that infects clinical Acinetobacter strains and represents a potential new candidate to be used in phage therapy. Electronic supplementary material The online version of this article (10.1007/s00705-019-04284-z) contains supplementary material, which is available to authorized users.
- Published
- 2019