1. A novel cetacean adenovirus in stranded harbour porpoises from the North Sea: detection and molecular characterization
- Author
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van Beurden, Steven J, IJsseldijk, Lonneke L, van de Bildt, Marco W G, Begeman, Lineke, Wellehan, James F X, Waltzek, Thomas B, de Vrieze, Geert, Gröne, Andrea, Kuiken, Thijs, Verheije, M Hélène, Penzes, Judit J, dPB I&I, dPB CR, LS Pathologie, Applied Veterinary Research, Veterinair Pathologisch Diagnostisch Cnt, Virology, Utrecht University [Utrecht], Erasmus University Medical Center [Rotterdam] (Erasmus MC), University of Florida [Gainesville] (UF), Institut Armand Frappier (INRS-IAF), Réseau International des Instituts Pasteur (RIIP)-Institut National de la Recherche Scientifique [Québec] (INRS), Harbour porpoise necropsies were funded by the Dutch Ministry of Economic Affairs (Grant Number 140000353)., This report was only possible thanks to the efforts made in finding and securing harbour porpoise carcasses by the volunteers of the Dutch stranding network, including the organizations and institutes involved. We thank Dr. Sal Frasca for histopathologic examination of the North American harbour porpoise., dPB I&I, dPB CR, LS Pathologie, Applied Veterinary Research, and Veterinair Pathologisch Diagnostisch Cnt
- Subjects
0106 biological sciences ,0301 basic medicine ,Bowhead Whale ,[SDV]Life Sciences [q-bio] ,Adenoviridae Infections ,Zoology ,Phocoena ,medicine.disease_cause ,010603 evolutionary biology ,01 natural sciences ,Adenoviridae ,Animal Diseases ,Protease Cleavage Site ,Feces ,03 medical and health sciences ,Phylogenetics ,Virology ,Primer walking ,medicine ,Animals ,Amino Acid Sequence ,14. Life underwater ,Phylogeny ,Intestinal Conten ,biology ,Phylogenetic tree ,Ecology ,Aquatic animal ,General Medicine ,biology.organism_classification ,Bottlenose dolphin ,Mastadenovirus ,030104 developmental biology ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Metagenomics ,North Sea ,Harbour Porpoise ,Bottlenose Dolphin - Abstract
International audience; Harbour porpoises (Phocoena phocoena) are the most prevalent cetaceans in the North Sea. The fecal viral flora of 21 harbour porpoises stranded along the Dutch coastline was analyzed by a metagenomics approach. Sequences of a novel cetacean mastadenovirus, designated harbour porpoise adenovirus 1 (HpAdV-1), were detected. The sequence of a 23-kbp genomic region, spanning the conserved late region, was determined using primer walking. Phylogenetic analysis indicated that HpAdV-1 is most closely related to bottlenose dolphin adenovirus and clusters with Cetartiodactyla adenoviruses. The prevalence of HpAdV-1 was low (2.6%) based on targeted PCR-screening of the intestinal contents of 151 harbour porpoises stranded between 2010 and 2013.
- Published
- 2017