1. Nitric oxide inhibits the replication cycle of porcine parvovirus in vitro
- Author
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Dong-Fang Wang, Hong-Ying Zhang, Bao-An Cui, Zhan-Yong Wei, Xue-Bin Wang, Hong-Ying Chen, Xiao-Dong Ning, and Yabin Wang
- Subjects
Porcine parvovirus ,Swine ,S-Nitroso-N-Acetylpenicillamine ,Arginine ,Nitric Oxide ,Virus Replication ,Severe Acute Respiratory Syndrome ,Antiviral Agents ,Nitric oxide ,Cell Line ,chemistry.chemical_compound ,Viral Proteins ,Virology ,Protein biosynthesis ,Japanese Encephalitis ,Animals ,Antiviral Effect ,biology ,Parvovirus ,Brief Report ,General Medicine ,Parvovirus, Porcine ,biology.organism_classification ,Molecular biology ,In vitro ,chemistry ,Viral replication ,Cell culture ,DNA, Viral ,DNA ,Japanese Encephalitis Virus - Abstract
This study investigated the inhibitory effect and mechanism of nitric oxide (NO) on porcine parvovirus (PPV) replication in PK-15 cells. The results showed that two NO-generating compounds, S-nitroso-l-acetylpenicillamine (SNAP) and l-arginine (LA), at a noncytotoxic concentration could reduce PPV replication in a dose-dependent manner and that this anti-PPV effect could be reversed by the NO synthase (NOS) inhibitor N-nitro-l-arginine methyl ester (l-NAME). By assaying the steps of the PPV life cycle, we also show that NO inhibits viral DNA and protein synthesis. This experiment provides a frame of reference for the study of the anti-viral mechanism of NO.
- Published
- 2008