1. Next generation sequencing and molecular analysis of artichoke Italian latent virus
- Author
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Toufic Elbeaino, Michele Digiaro, Imen Belghacem, Tiziana Mascia, and Donato Gallitelli
- Subjects
0301 basic medicine ,Polyproteins ,Nepovirus ,Artichoke italian latent virus ,DNA sequencing ,03 medical and health sciences ,Cynara scolymus ,Virology ,Amino Acid Sequence ,Grapevine chrome mosaic virus ,Phylogeny ,Plant Diseases ,chemistry.chemical_classification ,Genetics ,biology ,Phylogenetic tree ,High-Throughput Nucleotide Sequencing ,Sequence Analysis, DNA ,General Medicine ,biology.organism_classification ,Tomato black ring virus ,030112 virology ,Amino acid ,030104 developmental biology ,Italy ,chemistry - Abstract
Next-generation sequencing (NGS) allowed the assembly of the complete RNA-1 and RNA-2 sequences of a grapevine isolate of artichoke Italian latent virus (AILV). RNA-1 and RNA-2 are 7,338 and 4,630 nucleotides in length excluding the 3' terminal poly(A) tail, and encode two putative polyproteins of 255.8 kDa (p1) and 149.6 kDa (p2), respectively. All conserved motifs and predicted cleavage sites, typical for nepovirus polyproteins, were found in p1 and p2. AILV p1 and p2 share high amino acid identity with their homologues in beet ringspot virus (p1, 81% and p2, 71%), tomato black ring virus (p1, 79% and p2, 63%), grapevine Anatolian ringspot virus (p1, 65% and p2, 63%), and grapevine chrome mosaic virus (p1, 60% and p2, 54%), and to a lesser extent with other grapevine nepoviruses of subgroup A and C. Phylogenetic and sequence analyses, all confirmed the strict relationship of AILV with members classified in subgroup B of genus Nepovirus.
- Published
- 2017