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Start Over Journal archives of virology Region central african republic Publisher springer nature
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1. Usefulness of a genotypic resistance test using dried blood spot specimens in African HIV-infected children with virological failure according to the 2010-revised WHO criteria.

Author

Charpentier, Charlotte, Gody, Jean-Chrysostome, Tisserand, Pascaline, Matta, Mathieu, Fournier, Julien, Mbitikon, Olivia, and Bélec, Laurent

Subjects
HIV-positive children, ANTIRETROVIRAL agents, MICROBIAL mutation, RNA, VIROLOGY
Abstract

We compared paired plasma and dried blood spot (DBS) samples from 54 HIV-1-treated children living in Bangui, Central African Republic, for antiretroviral-resistance-associated mutations. All children displayed virological failure (HIV-1 RNA >3.70 logcopies/ml). Testing for resistance genotype was carried out in a reference laboratory in Paris, France. A successful test result was obtained in 54 (100%) plasmas and 25 DBSs (46%). Among the 732 resistance-associated mutations analyzed, 718 were identical, leading to a high concordance rate of 98.1%. Genotypic resistance tests on DBS samples were found to be highly feasible and accurate in a foreign reference laboratory, but with additional costs for shipping and decreased sensitivity. [ABSTRACT FROM AUTHOR]

Published
2011
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