1. Development of a TaqMan probe-based quantitative reverse transcription PCR assay for detection of Getah virus RNA.
- Author
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Shi, Ning, Liu, Hao, Li, Li-xia, Hu, Bo, Zhang, Lei, Zhao, Chuan-fang, Deng, Xiao-yu, Li, Xin-tong, Xue, Xiang-hong, Bai, Xue, Zhang, Hai-ling, Lu, Rong-guang, Lian, Shi-zhen, Wang, Yang, Yan, Ming-hao, and Yan, Xi-jun
- Subjects
VIRAL disease diagnosis ,VIRAL genetics ,REVERSE transcriptase polymerase chain reaction ,BIOLOGICAL assay ,POLYMERASE chain reaction ,MOLECULAR probes - Abstract
Getah virus (GETV), a mosquito-borne virus that mainly infects horses and pigs, has emerged and spread in China. We developed a highly specific and reproducible TaqMan probe-based quantitative reverse transcription PCR (RT-qPCR) assay targeting the non-structural protein 1 of GETV, whose detection limit is 25.5 copies/µL, which is 100-fold higher than that of conventional RT-PCR. RT-qPCR was used to detect GETV RNA in mosquito and animal clinical samples, showing that the accuracy of RT-qPCR was higher than that of conventional RT-PCR. The newly developed RT-qPCR assay may be a useful alternative tool for rapid, simple and specific diagnosis of GETV infection. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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