1. Mesorhizobium amorphae, a rhizobial species that nodulates Amorpha fruticosa, is native to American soils.
- Author
-
Wang ET, Rogel MA, Sui XH, Chen WX, Martínez-Romero E, and van Berkum P
- Subjects
- China, DNA, Ribosomal analysis, Electrophoresis methods, Iowa, Isoenzymes analysis, RNA, Ribosomal, 16S genetics, Rhizobiaceae genetics, Sequence Analysis, DNA, Symbiosis, Fabaceae microbiology, Nitrogen Fixation, Plant Roots microbiology, Rhizobiaceae classification, Rhizobiaceae isolation & purification, Soil Microbiology
- Abstract
Amorpha fruticosa was inoculated with rhizosphere soil from Iowa, USA, and 140 rhizobia isolated from root nodules were compared with Mesorhizobium amorphae originating from Chinese soils. PCR-RFLP patterns of the 16S rRNA gene from the isolates and from M. amorphaewere the same. All isolates had a symbiotic plasmid of the same size with a single nifHgene. DNA:DNA hybridization values, DNA G+C content, and induced Nod factor patterns also were similar. We concluded that the four genotypes distinguished among 53 representative American isolates were M. amorphae. Since A. fruticosa is native to the Americas and is highly specific in its nodulation requirement, M. amorphae probably was transmitted to China.
- Published
- 2002
- Full Text
- View/download PDF