1. Multidrug-resistant Raoultella ornithinolytica misidentified as Klebsiella oxytoca carrying blaOXA β-lactamases: antimicrobial profile and genomic characterization
- Author
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Juan Carlos Bravata-Alcántara, Noé Escobar-Escamilla, Fabiola Garcés-Ayala, Juan Manuel Bello-López, Adnan Araíza Rodriguez, Julio Cesar Juárez-Gómez, Víctor Hugo Gutiérrez-Muñoz, Iliana Alejandra Cortés-Ortíz, Edgar Mendieta-Condado, and José Ernesto Ramírez–González
- Subjects
medicine.drug_class ,Cephalosporin ,Microbial Sensitivity Tests ,Biochemistry ,Microbiology ,beta-Lactamases ,Antibiotic resistance ,Bacterial Proteins ,Enterobacteriaceae ,polycyclic compounds ,Genetics ,medicine ,Molecular Biology ,biology ,Klebsiella oxytoca ,General Medicine ,Genomics ,biochemical phenomena, metabolism, and nutrition ,Plasmid-mediated resistance ,biology.organism_classification ,Antimicrobial ,Raoultella ornithinolytica ,Anti-Bacterial Agents ,Penicillin ,Multiple drug resistance ,bacteria ,medicine.drug - Abstract
Class D β-lactamases OXA-232 and OXA-48 hydrolyze penicillin, cephalosporins and carbapenems, limiting the pharmacological therapeutics in bacteraemia. OXA producer microorganisms are considered a great emergent threat, especially in nosocomial environments. To determine the resistance profile and genomic characterization of two isolates initially identified as potential carbapenemase-producer Klebsiella oxytoca in a third level hospital. Automated platform BD Phoenix-100 System was used to identify and to biochemically characterize both isolates. Furthermore, the resistance profile was determined through CLSI methods and the whole genome sequences were obtained using Next-Generation Sequencing. Resistance genes were analyzed, and the virtual fingerprinting was determined to corroborate the similarity with related bacteria. Both strains correspond to Raoultella ornithinolytica carrying OXA 232 and OXA-48 genes, confirming the class D β-lactamases assay results. Here, we present the genetic and phenotypic analysis of multidrug resistance R. ornithinolytica, representing the first report in Mexico.
- Published
- 2021