1. Does sildenafil have protective effects against ovarian ischemia-reperfusion injury in rats?
- Author
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Incebiyik, Adnan, Seker, Ahmet, Camuzcuoglu, Hakan, Kocaslan, Sezen, Camuzcuoglu, Aysun, Hilali, Nese, Vural, Mehmet, Taskin, Abdullah, and Aksoy, Nurten
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SILDENAFIL , *OVARIAN diseases , *REPERFUSION injury , *OXIDATIVE stress , *LABORATORY rats - Abstract
Purpose: The aim of this study was to evaluate the protective activity of sildenafil treatment against ischemia-reperfusion damage created experimentally in rat ovaries. Methods: For this study, 42 female Wistar rats were used, and the rats were separated randomly into six groups consisting of seven rats each: sham, torsion, torsion-detorsion, torsion-detorsion + saline, torsion-detorsion + sildenafil 0.7 mg/kg and torsion-detorsion + sildenafil 1.4 mg/kg. With the exception of the sham group, an ovarian torsion procedure was implemented in all other groups for 2 h. Then, a detorsion procedure was implemented to the groups for 2 h, with the exception of the torsion group. Medications were given intraperitoneally, one-half hour before the detorsion procedure in the saline, 0.7 and 1.4 mg/kg sildenafil groups. Finally, 2 ml of blood samples was drawn for markers of oxidative stress, while the ovaries which were torsioned for the histological examination were extracted from all rats. Results: According to the histopathological damage scores, the least damage was seen in the sham group and the most damage was seen in the torsion-detorsion group. The sildenafil treatment appeared to be effective in decreasing tissue damage; however, there were no differences between the dosages. Additionally, it was determined that the oxidative stress levels were higher in the torsion-detorsion group, while the sildenafil treatment caused a significant decrease in the oxidative stress levels. Conclusions: The results of the current study showed that the sildenafil treatment can be effective in preventing tissue damage and oxidative stress induced by the ischemia-reperfusion created in rat ovaries. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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