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2 results on '"Voldemar Toome"'

1. Colorimetric amino acid analysis using fluorescamine

Author

Voldemar Toome, Manfred Weigele, Arthur Felix, and Silvano De Bernardo

Subjects
Chemical Phenomena, Proline, Glycine, Biophysics, Peptide, Fluorescamine, Biochemistry, chemistry.chemical_compound, Amino acid analysis, Fluorometry, Spiro Compounds, Amino Acids, Molecular Biology, chemistry.chemical_classification, Chromatography, Chromophore, Chromatography, Ion Exchange, Fluorescence, Amino acid, Chemistry, chemistry, Spectrophotometry, Colorimetry, Peptides, Quantitative analysis (chemistry)
Abstract

Fluorescamine reacts efficiently with primary and secondary amino acids to form fluorescent pyrrolinone and nonfluorescent aminoenone type chromophores, respectively. A procedure is described for quantitative colorimetric determination at one fixed wavelength of the full array of natural amino acids, including proline and its derivatives. Simultaneous colorimetric-fluorometric analysis enables distinction between primary and secondary amino acids and permits their quantitative analysis with fluorescamine over a broad concentration range. These methods can also be applied for peptide analyses.

Published
1975
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2. Studies on the reaction of fluorescamine with primary amines

Author

Silvano De Bernardo, Manfred Weigele, Sidney Udenfriend, Peter Böhlen, Willy Leimgruber, Stanley Stein, Voldemar Toome, and Karin Manhart

Subjects
Magnetic Resonance Spectroscopy, Spectrophotometry, Infrared, Kinetics, Biophysics, Glycine, Molecular Conformation, Fluorescamine, Biochemistry, chemistry.chemical_compound, Structure-Activity Relationship, Leucine, Spectrophotometry, medicine, Animals, Dimethyl Sulfoxide, Amines, Molecular Biology, Fluorescent Dyes, Primary (chemistry), Chromatography, Alanine, Aniline Compounds, Binding Sites, medicine.diagnostic_test, Dimethyl sulfoxide, Serum Albumin, Bovine, Stereoisomerism, Dipeptides, Hydrogen-Ion Concentration, Fluorescence, Spectrometry, Fluorescence, chemistry, Reagent, Quantum Theory, Amine gas treating, Cattle, Indicators and Reagents, Spectrophotometry, Ultraviolet, Protein Binding
Abstract

Fluorescamine is a useful reagent for the fluorometric assay of primary amines. The extent of the reaction between fluorescamine and primary amines, as well as the fluorescence intensities of the resulting fluorophors depend on pH, solvent composition and reagent concentration. Optimum values for these variables further depend on the amine under study. The influence of these parameters on the fluorogenic reaction of representative amines, and on their fluorophoric derivatives has been investigated, and the results are reported here.

Published
1974

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