1. Oridonin suppresses the growth of glioblastoma cells via inhibiting Hippo/YAP axis.
- Author
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Wang, Chen, Zhang, Yonghong, Jiang, Qingsong, Chen, Shuang, Zhang, Liang, and Qiu, Hongmei
- Subjects
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YAP signaling proteins , *CELL growth , *NUDITY , *BRAIN tumors , *INHIBITION of cellular proliferation , *GLIOBLASTOMA multiforme - Abstract
Glioma is a brain tumor that originates from brain or spine glial cells. Despite alternative treatments, the overall survival rate remains low. Oridonin (ORI) is purified from the Chinese herb Rabdosia rubescens, which has exhibited positive effects on tumors. This study aimed to investigate the effect of ORI on U87MG glioblastoma cells and whether the Hippo/YAP-related signaling pathway was involved. Malignant glioblastoma U87MG cells and male athymic nude mice (BALB/cnu/nu) were used as the experimental models. The YAP inhibitor Verteporfin (VP) and the overexpression of YAP were used to investigate its potential relation with glioma. Here, we found that ORI inhibited cell proliferation and promoted cell apoptosis in a dose-dependent manner in U87MG cells. Moreover, ORI inhibited Bcl-2, YAP, and c-Myc protein expression but increased Bax, caspase-3, and p-YAP protein expression. Furthermore, the effect of ORI was also confirmed in a mouse model bearing glioma. ORI reversed the effect of overexpression of YAP. Collectively, oridonin suppressed glioblastoma oncogenesis via the Hippo/YAP signaling pathway and could be a potential therapeutic target in the treatment of glioblastoma. [Display omitted] • Oridonin suppressed glioblastoma oncogenesis in U87MG glioblastoma cells. • Oridonin suppressed glioblastoma oncogenesis growth in mouse model bearing glioma. • Oridonin suppressed glioblastoma oncogenesis via the Hippo/YAP signaling pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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