1. The effect of epidermal growth factor receptor variant III on glioma cell migration by stimulating ERK phosphorylation through the focal adhesion kinase signaling pathway
- Author
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Liu, Mingzhu, Yang, Yong, Wang, Can, Sun, Lidong, Mei, Chuanzhong, Yao, Wantong, Liu, Yonglei, Shi, Yinghong, Qiu, Shuangjian, Fan, Jia, Cai, Xiumei, and Zha, Xiliang
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EPIDERMAL growth factor , *CELL migration , *CELLULAR signal transduction , *PHOSPHORYLATION , *FOCAL adhesion kinase , *GLIOBLASTOMA multiforme , *CANCER cells , *GENETIC mutation - Abstract
Abstract: Epidermal growth factor receptor variant III (EGFRvIII), the most common EGFR mutation, is associated with cell migration of glioblastoma multiforme (GBM) cases; however, the mechanism has not been elucidated. In this study, we found that the EGFRvIII-promoted glioma cell migration was closely linked to high levels of tyrosine phosphorylation in focal adhesion kinase (FAK) Y397. We also demonstrated that EGFRvIII formed a complex with FAK, resulting in enhanced tyrosine phosphorylation levels of FAK Y397 and EGFR Y1068. After knockdown of FAK expression via anti-FAK shRNA, the U87ΔEGFR cell migration was significantly inhibited, accompanying with the reduced phosphorylation levels of extracellular signal-regulated kinase (ERK1/2). Furthermore, the role of ERK1/2 in FAK-regulated cell migration was confirmed. Taken together, our results suggest that FAK and its downstream molecule ERK were involved in EGFRvIII-promoted glioma cell migration in U87ΔEGFR cells. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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