Search

Background: SLC26A4 gene mutations are the second identifiable genetic cause of autosomal recessive non-syndromic hearing loss (ARNSHL) after GJB2 mutations. In this study, the identity and informativeness of D7S2459 marker in SLC26A4 gene region was examined in five ethnic groups of the Iranian population. Materials and Methods: The study was accomplished by genotyping the locus in 165 individuals of five different ethnic groups including Fars, Azari, Torkaman, Gilak and Arab using polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis (PAGE) and fluorescent capillary electrophoresis. In this study, results were analyzed by GeneMarker HID Human STR Identity software, GenePop program and Microsatellite Tools software. Results: Analysis of the allelic frequency revealed the presence of 8 alleles of D7S2459 marker in the Iranian population. Among all, allele 148bp at the D7S2459 locus was the most frequent with 31% frequency. The most heterozygosity observed in Azari ethnic by 84.8%. Finally, analysis of PIC value revealed that D7S2459 marker could be considered as a highly informative marker in each ethnic of the Iranian population (PIC value above 0.7). Conclusion: Our data suggested that D7S2459 could be introduced as a highly informative marker in molecular diagnosis of SLC26A4 based ARNSHL by Linkage analysis. [ABSTRACT FROM AUTHOR]