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In order to explore the changes in protein expression of large yellow croaker Larimichthys crocea under high temperature stress, isobaric tags for relative and absolute quantitation (iTRAQ) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to perform proteome analysis on the liver between thermal-tolerant and thermal-sensitive groups of large yellow croaker. A total of 23,269 peptides and 3,388 proteins were identified, and 442 differentially expressed proteins (DEPs) were screened, among which 171 proteins were up-regulated and 271 proteins were down-regulated with fold change ≥ 1.2 or

Fish skin mucus serves as the first line of defense against pathogens and external stressors. To screen thermal-tolerant protein marker in the skin mucus of large yellow croaker Larimichthys crocea, this study conducted proteome analysis on skin mucus between thermal-tolerant and thermal-sensitive groups of large yellow croaker. A total of 396 differentially expressed proteins (DEPs) were identified, among which 215 proteins were upregulated and 181 proteins were downregulated. Ten selected random DEPs were validated using a parallel reaction monitoring (PRM) assay, and the results showed that the proteome analysis results were reliable (r = 0.805, P

At present, the methods for monitoring the health status of fish mostly utilize blood biochemical indicators. As the blood collection process often causes damage to the fish body (sometimes causing death), the need to develop a noninvasive detection method is urgent. Previous studies have shown that stress indicators can be detected in fish skin mucus or the surrounding water. In this experiment, the level of cortisol in the blood, fish skin mucus, and surrounding water of large yellow croaker was determined at 0, 0.5, 1, 1.5, and 2 h after high-temperature stress induction. Moreover, the levels of malondialdehyde (MDA), immunoglobulin M (IgM), and alkaline phosphatase (AKP) in the serum and fish skin mucus were also measured. The results were as follows: with increasing stress duration, the cortisol levels in the serum, fish skin mucus, and surrounding water increased first and then decreased, all reaching a peak at 1.5 h. The Pearson correlation coefficients for the cortisol levels between the serum and skin mucus, serum and surrounding water, and skin mucus and surrounding water were 0.936, 0.955, and 0.915, respectively, all with a significance level of P < 0.01. The MDA and IgM levels in the serum and fish skin mucus both increased first and then decreased, while the AKP levels in the serum and skin mucus decreased first and then increased. For the MDA, IgM, and AKP levels, the Pearson correlation coefficients between the serum and skin mucus were 0.586, 0.762, and 0.792, respectively, all with a significance level of P < 0.01. According to the results mentioned above, we can use the surrounding water or skin mucus as media to monitor early stress responses in large yellow croaker under high-temperature stress.