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This study aimed to determine the causative agent of black spots which observed in the mesentery, adipose tissue and peritoneum of orange-spotted grouper, Epinephelus coioides. The parasitological examination using light and electron microscope confirmed infection with trichosomoidid parasite, genus Huffmanela, which identified as Huffmanela japonica (H. japonica). The examined eggs were present in two stages (stage 1, mature light brown eggs with larva; stage 2, advanced dark brown eggs with grown larva). The histopathological alteration of the infected tissues together with determination of immunological gene expression in different organs demonstrated prominent response of the host tissues against the studied parasite. Pathological examination of the infected fish revealed that the lesions were mainly in the mesenteric and diffuse pancreatic tissues which present in the abdominal fat. Granulomas containing eggs and mononuclear and eosinophilic granular cell infiltrations, together with fat necrosis of the pancreatic tissue, were the most common pathological findings. Migrating larvae and peritoneal adhesions with organised fibrosis were also observed. In the infected tissues as well as in the spleen, liver and kidney, interleukin 1β (IL-1β) and cyclooxygenase (COX)-2 were upregulated than in control non-infected fish. Our results confirmed that H. japonica is infecting orange-spotted grouper caught from the Red Sea in Egypt with prominent tissue specificity in the mesentery, adipose tissue surrounding the intestine, and in the peritoneum. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the presence of Sarcotaces sp. in Epinephelus epilistictus (the dotted grouper) in Saudi Arabia. So, during our routine clinical examination of fish health conditions at Jubail province in the eastern region of Saudi Arabia (Arabian Gulf), nine grouper fish species were examined for Sarcotaces sp. (Copepoda: Phylichthyidae). Only the dotted grouper (Epinephelus epilistictus) harbored parasitic Sarcotaces spp. with an incidence of 9.06% of 1600 clinically examined fish samples. The collected Sarcotaces sp. was Sarcotaces arcticus (S. arcticus). The copepods were completely encapsulated cysts with a pyriform or pear-like shape, small to large, white-grayish, fluid-filled with thick black ink beneath the skin, or fully embedded in the muscular layer. The cysts present in the lateral aspect of trunk muscles, flank, caudal peduncle, near the anal opening, or pelvic and pectoral fin with an infestation rate (1–6 cysts) per fish. The cyst histopathology reveals the capsular layer of the cyst consisting of fibrous connective with pressure atrophy of the adjacent muscles associated with dilated blood vessels. The absence of infestation in other kinds of grouper fish species from the water of the Arabian Gulf in Jubail province may indicate parasitic host specificity and geographic locality distribution. [ABSTRACT FROM AUTHOR]

This document is a correction notice for an article titled "Heavy infection of the orange-spotted grouper (Epinephelus coioides) with Hufmanela japonica: morphological, ultrastructural identification, tissue reactions and immunological analysis" published in Aquaculture International. The correction involves the replacement of Figure 5 in the original article with a new version that has higher resolution and a minor modification to the image. The corrected Figure 5 is included in the correction notice. The publisher, Springer Nature, maintains a neutral stance on jurisdictional claims and institutional affiliations. The authors of the original article are Marwa M. Attia, Mustafa M. Ibrahim, and Mahmoud A. Mahmoud. [Extracted from the article]