1. Identification of novel Z/W chromosome-specific markers from the giant freshwater prawn Macrobrachium rosenbergii.
- Author
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Xue Liu, Lingxia Zhou, Biyun Luo, Hongli Qian, Baoqing Ye, Keyi Ma, and Gaofeng Qiu
- Subjects
MACROBRACHIUM rosenbergii ,SEX chromosomes ,POLYMERASE chain reaction ,NUCLEOTIDES - Abstract
The giant freshwater prawn (Macrobrachium rosenbergii) is a commercially cultured species in China. A sex chromosome-specific marker is crucial for sex identification and distinguishing sex chromosomes. Here, based on the results of reduced-representation genome sequencing and genome survey of M. rosenbergii, we identified steady sex-linked markers. Further nucleotide alignment analysis revealed 83.42% nucleotide similarity between the amplified W- and Z-chromosome fragments. qPCR confirmed that Z chromosome-specific fragments were present in males approximately twice as often compared to females. Similarly, the W chromosome-specific fragments were present in super females approximately twice as often compared to females. Our findings elucidate the ZW/ZZ sex determination type and contribute to exploring sex-related or sex-determining genes present on the sex chromosomes of M. rosenbergii. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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