1. Activation of immunological responses in Litopenaeus setiferus hemocytes by a hemocyanin like-lectin
- Author
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Juan Alpuche, Edgar Zenteno, Cristina Pascual, Jorge Guevara, Ali Pereyra, Concepción Agundis, Carlos Rosas, and Lorena Vázquez
- Subjects
medicine.medical_treatment ,Lectin ,Hemocyanin ,Oxidative phosphorylation ,Aquatic Science ,Biology ,Respiratory burst ,Superoxide dismutase ,Immune system ,Biochemistry ,Humoral immunity ,medicine ,biology.protein ,Receptor - Abstract
Article history:Received 6 February 2008Received in revised form 19 March 2009Accepted 19 March 2009Keywords:HemocyaninReceptorLectinCrustaceanLitopenaeus setiferusOxidative burstN-acetylated carbohydratesReactive oxygen intermediates Inordertoidentifythespecificroleofhumorallectinsincrustaceans'immuneresponse,weevaluatedtheeffectofthe 291 kDa hemocyanin-like lectin from Litopenaeus setiferus (LsL) on its own hemocytes. LsL binds 5.6% ofcirculatingnon-activatedhemocytes,butisabletorecognize85%oftotaladhered(activated)hemocytes,showingfilipodiaandpseudopodia,inducingtheproductionofreactiveoxygenintermediates(ROIs)inatime-anddose-dependent manner (100 ng/10 5 cells/20 min). This effect was inhibited specifically by N-acetylated sugars(NeuAc, GalNAc or GlcNAc), as well as by superoxide dismutase (SOD), acetylsalicylic, and mefenamic acid. Inhemocytes, LsL also induced higher ROIs production than Zymozan (pN0.05). The receptor for LsL in thehemocytesmembraneisaglycoproteinof61and52kDapersubunit,asdeterminedbywesternblot.Ourresultssuggest that LsL participates in the activation of oxidative immune responses of granularhemocytes induced byNADPH and other oxidative pathways through a specific membrane receptor.© 2009 Elsevier B.V. All rights reserved.
- Published
- 2009