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The digital twinning process is essential for transferring real-world objects to the Metaverse by creating accurate digital versions, known as digital twins. However, complex systems pose challenges in this process. With the increasing utilization of microwave components and circuits in telecommunication systems such as IoT, 5 G, and 6 G, the need for digital twins of these components arises. Nevertheless, high-frequency components exhibit intricate behavior, requiring advanced modeling techniques. Artificial intelligence (AI) provides a powerful tool for enhancing the reliability and accuracy of estimated models in such cases. In this study, a microstrip lowpass filter (LPF) is designed, fabricated, and measured as the physical twin. An intelligent digital twinning approach is employed using a machine learning method based on an adaptive neuro-fuzzy inference system (ANFIS), trained by a subtractive clustering algorithm. The resulting digital twin of the LPF proves valuable for communication networks and IoT applications. Moreover, this research showcases the applicability and accessibility of machine learning in creating digital twins of electromagnetic components for communication cyber-physical systems (CPSs) and the Metaverse. Furthermore, the proposed method exhibits adaptability to various passive and active electrical or electronic circuits. By harnessing the potential of AI and digital twinning, this study presents a robust and accurate approach for modeling and analyzing complex circuits, specifically in the context of communication systems and their integration into the Metaverse. The findings highlight the advantages of an intelligent digital twinning approach and its potential for advancing various domains involving complex circuitry. • Machine learning for accurate digital twins in telecom & Metaverse. • Microstrip filter case study shows IoT & network benefits. • Machine learning enhances electromagnetic twins for CPS & Metaverse. • AI-driven circuit modeling & analysis advances in telecom. • Robust digital twin solutions for IoT & future 5G/6G tech. [ABSTRACT FROM AUTHOR]

Preparing the infrastructure for analyzing, recognizing, and characterizing microorganisms in the Metaverse can transform the fields of biology, medicine, and drug discovery. Accordingly, the realization of digital twins of microorganisms, such as viruses, fungi, algae, bacteria, protozoa, archaea, and multicellular animal parasites, can streamline the applicability of the Metaverse and similar emerging technologies like Cyber-Physical Healthcare Systems (CPHS). This is why a new approach to the digital twinning of bacteria has been presented in this research. This method of digital twinning can revolutionize the research and study of bacteria because it allows us to separate useful and harmful bacterial species, increasing the efficiency of treatment noticeably. This innovative method can easily be used by clinics, medical centers, or even by private users physically and virtually and can be adapted to every centralized or decentralized Metaverse Platform. To determine the proper treatment, biologists have always tried to identify the correct bacterial species that prompted a bacterial infection. They use various indicators, such as the bacterial cell's shape and the size of the colony formed by the bacteria, to classify different types of bacteria with different biochemistries and shapes. However, it is challenging because of the extensive similarities between some species. For instance, such similarities exist between Staphylococcus aureus and Staphylococcus saprophyticus, which has caused numerous false diagnostic reports by operators. Wrong species reports bring about treatment failure and increase antibiotic resistance issues. Therefore, the digital twins of bacteria cover all of their identifiable characteristics and overcome many limitations to study them. In this approach, DTL techniques, including MobileNetV2, EfficientNetV2-S, and ResNet-50, have been employed to build digital twins of bacteria species. A hybrid dataset was used for training and evaluation. Among the models, EfficientNetV2-S exhibited the best performance, with a validation accuracy of 99.58% and a test accuracy of 99.33%. The results showed the ability of deep learning models to make bacteria digital twins based on image processing from different labs, thus assisting experts in speeding up the process and reducing diagnostic errors. In addition, in the mispredicted cases, the correct species was among the first three choices of the model. Therefore, not only can experts use DTL approaches to speed up the digital twin realization of microorganisms in the Metaverse, but they can also use these methods to reduce diagnostic errors. • Introducing a technique to streamline digital twinning of microorganisms using CNN. • Describing machine learning digital twins of microorganisms in the Metaverse. • Presenting an exact, economical digital twinning approach via smartphone photography. • Providing a comprehensive bacteria species dataset for future method development. [ABSTRACT FROM AUTHOR]