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Variations in illumination and image background present challenges for using UAV RGB imagery. Existing studies often overlook these issues, especially in rice. To separately evaluate the impacts of illumination variation and image background on rice LAI assessment, this study utilized Retinex correction and image segmentation to eliminate illumination variations and background effects, and then analyzed the changes in color indices and their relationship with LAI before and after implementing these methods separately. The results indicated that both Retinex correction and image segmentation significantly enhanced the correlation between color indices and LAI at different growth stages as well as the accuracy of constructing a multivariate linear regression model separately. Our analysis confirmed the significance of accounting for variation in illumination and rice field backgrounds in LAI analysis when using UAV RGB images. Illumination variation and image background elements significantly degrade the accuracy of LAI estimation. [ABSTRACT FROM AUTHOR]