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We have developed a pulsed Yb:YAG thin disk green laser for pumping a 100 kHz and 10 W femtosecond laser. We optimized numerically and experimentally the open duration of the acousto-optic (AO) Q-switcher and the internal SHG method, generating stable output energy at a 100 kHz repetition rate. We demonstrated a 515 nm output power of over 80 W with a pulse-to-pulse stability of less than 1% RMS with an LD power to green power conversion efficiency of 24%. With the developed green laser, we obtained 16 W of a Ti:sapphire laser in a cavity dumped mode.

We have developed a 6–12 μm mid-infrared (MIR) femtosecond laser source for glyco-protein structure analysis. The MIR femtosecond laser pulses are generated by a differential frequency generation (DFG) configuration with a combination of Ti:sapphire based regeneratively amplified femtosecond laser pulses (780 nm, 160 fs, 1 mJ) and a β-BaB2O4 (BBO) based optical parametric amplifier (OPA). The MIR pulse energy exceeds 4.5 μJ, where a glyco-protein molecule has resonant absorption lines due to the vibrational–rotational transitions. The pulse width is estimated to be less than 1 ps according to the cross correlation measurement between the two OPA output pulses. Using the MIR femtosecond laser pulses, we demonstrated photo-dissociation of the sialyl Lewis X (sLeX) proton added ion, which is the first time to the best of our knowledge.