Search

We describe a new technique for differential interference contrast (DIC) microscopy, which digitally generates phase gradient images independently of gradient orientation. To prove the principle we investigated specimens recorded at different orientations on a microscope equipped with a precision rotating stage and using regular DIC optics. The digitally generated images successfully displayed and measured phase gradients, independently of gradient orientation. One could also generate images showing distribution of optical path differences or enhanced, regular DIC images with any shear direction. Using special DIC prisms, one can switch the bias and shear directions rapidly without mechanically rotating the specimen or the prisms and orientation-independent DIC images are obtained in a fraction of a second. OCIS codes: 180.0180, 100.2980, 170.1530, 170.3010, 170.3890, 170.3880.

We propose image processing algorithms for measuring two-dimensional distributions of linear birefringence using a pair of variable retarders. Several algorithms that use between two and five recorded frames allow us to optimize measurements for speed, sensitivity, and accuracy. We show images of asters, which consist of radial arrays of microtubule polymers recorded with a polarized light microscope equipped with a universal compensator. Our experimental results confirm our theoretical expectations. The lowest noise level of 0.036 nm was obtained when we used the five-frame technique and four-frame algorithm without extinction setting. The two-frame technique allows us to increase the speed of measurement with acceptable image quality. OCIS codes: 180.0180, 260.1440, 260.2130, 260.5430, 170.1530, 120.5410.

An optical fiber refractometer based on a photometric return-path birefringence sensor is proposed. For measuring the refractive index, the phase shift between polarization components on total internal reflection inside a refractometric prism is used. Several kinds of refractometric prism are described. It is shown that a refractive-index sensitivity of 0.0001 and higher for a wide range of index values is attainable. [C] 2001 Optical Society of America OCIS codes: 060.2370, 120.2130, 120.5710, 260.1440, 260.2130, 260.6970.