Search

The glucoamylase gene of the yeast Arxula adeninivoranswas expressed in Kluyveromyces lactisby using the GAPpromoter from Saccharomyces cerevisiaeand a multicopy plasmid vector. The transformants secreted 90.1% of the synthesized glucoamylase into the culture medium. The secreted glucoamylase activities are about 20 times higher in comparison to those of Saccharomyces cerevisiaetransformants using the same promoter. Secreted glucoamylase possesses identical N-terminal amino acid sequences to those secreted by A. adeninivoransshowing that cleavage of the N-terminal signal peptide takes place at the same site. Biochemical characteristics of glucoamylase expressed by K. lactisand A. adeninivoransare very similar.