1. Diversity and enrichment of nitrite-dependent anaerobic methane oxidizing bacteria from wastewater sludge.
- Author
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Luesken FA, van Alen TA, van der Biezen E, Frijters C, Toonen G, Kampman C, Hendrickx TL, Zeeman G, Temmink H, Strous M, Op den Camp HJ, and Jetten MS
- Subjects
- Anaerobiosis, Bacterial Proteins genetics, Cluster Analysis, DNA, Bacterial chemistry, DNA, Bacterial genetics, DNA, Ribosomal chemistry, DNA, Ribosomal genetics, Methylococcaceae genetics, Methylococcaceae metabolism, Molecular Sequence Data, Netherlands, Phylogeny, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Methane metabolism, Methylococcaceae isolation & purification, Nitrites metabolism, Sewage microbiology
- Abstract
Recently discovered microorganisms affiliated to the bacterial phylum NC10, named "Candidatus Methylomirabilis oxyfera", perform nitrite-dependent anaerobic methane oxidation. These microorganisms could be important players in a novel way of anaerobic wastewater treatment where ammonium and residual dissolved methane might be removed at the expense of nitrate or nitrite. To find suitable inocula for reactor startup, ten selected wastewater treatment plants (WWTPs) located in The Netherlands were screened for the endogenous presence of M. oxyfera using molecular diagnostic methods. We could identify NC10 bacteria with 98% similarity to M. oxyfera in nine out of ten WWTPs tested. Sludge from one selected WWTP was used to start a new enrichment culture of NC10 bacteria. This enrichment was monitored using specific pmoA primers and M. oxyfera cells were visualized with fluorescence oligonucleotide probes. After 112 days, the enrichment consumed up to 0.4 mM NO(2)(-) per day. The results of this study show that appropriate sources of biomass, enrichment strategies, and diagnostic tools existed to start and monitor pilot scale tests for the implementation of nitrite-dependent methane oxidation in wastewater treatment at ambient temperature., (© The Author(s) 2011. This article is published with open access at Springerlink.com)
- Published
- 2011
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