1. Genetically Engineered Saccharomyces cerevisiae Strain that Can Ultilize Both Xylose and Glucose for Fermentation
- Author
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Gang Song, Lu Yan Zhang, Jing Ping Ge, Meng Yun Zhang, Yan Shen, and Wen Xiang Ping
- Subjects
biology ,Strain (chemistry) ,Chemistry ,Saccharomyces cerevisiae ,General Medicine ,Xylose ,biology.organism_classification ,Transformation (genetics) ,Metabolic pathway ,chemistry.chemical_compound ,Plasmid ,Biochemistry ,Yield (chemistry) ,Fermentation - Abstract
The primary problem in producing fuel ethanol through microorganism fermentation with lignocellulose is the strain. We constructed a URA3-directed low copy integration-expression plasmid pZMYBX1 and rDNA-directed high copy integration-expression plasmid pZMYX2. Using the lithium acetate transformation method, we co-transformed the linearized plasmid pZMYBX1 (StuI) and pZMYX2 (HpaI) into theS. cerevisiaecells. Ultimately, we obtain three recombinants: HDY-ZMYWBG1, HDY-ZMYWBG2 and HDY-ZMYWBG3. The ethanol yield for HDY-ZMYWBG1 and HDY-ZMYWBG3 are 0.368 g/g and 0.365 g/g, respectively, which are higher than the 0.330 g/g yield for W5. This findings show that the xylose metabolic pathway could be introduced into theS. cerevisiaeto produce an alternative strain for the production of biological ethanol from lignocellulose substrate.
- Published
- 2013
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