1. Isolation, Structure Identification and Cytotoxicity Evaluation of Three Steroids from Boletus edulis
- Author
-
Qi Lan Liang
- Subjects
Stigmasterol ,biology ,Traditional medicine ,General Medicine ,Fractionation ,Fungus ,Isolation (microbiology) ,biology.organism_classification ,chemistry.chemical_compound ,chemistry ,Boletus edulis ,Ic50 values ,Cytotoxicity ,Human cancer - Abstract
BEL-7402, HT-29, SPC-A1, U-251 bioassay-guided fractionation led to the isolation of the three active constituents from a wild fungi, Boletus edulis Bull. Ex Fr., which collected from Changbai Montain area. These three compounds’ chemical structures were elucidated on the base of spectroscopic data and comparison with known compounds as 5-cholestene-2,3-oxide (1), β-sitosterol (2), stigmasterol (3). All of the three compounds were extracted from Boletus edulis for the first time, and compound 1 was significantly cytotoxic against the four human cancer cell lines (BEL-7402, HT-29, SPC-A1 and U-251) with IC50 values 6.29, 6.98, 4.79 and 7.54 μM, respectively. Results of the study could supply referencs to the people to find the new drug from the fungus.
- Published
- 2014