1. Tangeretin synergistically enhances odorant-induced increases in cAMP and calcium levels and CREB phosphorylation in non-neuronal 3T3-L1 cells
- Author
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Jae-Ho Park, Jin-Taek Hwang, Mi Jeong Sung, Myung-Sunny Kim, Haeng Jeon Hur, Yeo Cho Yoon, Sung Hee Kim, and Mee-Ra Rhyu
- Subjects
0301 basic medicine ,biology ,Organic Chemistry ,3T3-L1 ,Stimulation ,CREB ,General Biochemistry, Genetics and Molecular Biology ,Cell biology ,Adenylyl cyclase ,03 medical and health sciences ,Tangeretin ,chemistry.chemical_compound ,030104 developmental biology ,0302 clinical medicine ,chemistry ,Biochemistry ,biology.protein ,Phosphorylation ,Cyclic adenosine monophosphate ,Signal transduction ,030217 neurology & neurosurgery - Abstract
Tangeretin is a natural flavone found mainly in citrus peels. Although emerging evidence suggests the beneficial health effects of tangeretin, little is known about the biological role of tangeretin in the odorant-induced signal transduction pathway. In this study, the effects of tangeretin on odorant-stimulated non-neuronal 3T3-L1 cells were evaluated. Here, we present the first evidence that an olfactory receptor for lyral, an odorant, is expressed in 3T3-L1 cells and that it responds to its ligand. Stimulation with lyral increased the cyclic adenosine monophosphate (cAMP) and Ca2+ levels as well as the phosphorylation of cAMP response element binding protein (CREB). Pretreatment with tangeretin synergistically enhanced the effects of stimulation with lyral: up-regulation of cAMP and Ca2+ levels and CREB phosphorylation. These data collectively suggest that tangeretin synergistically enhances the odorant-induced signaling pathway through modulation of cAMP and CREB signal transduction in non-chemosensory cells.
- Published
- 2016
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