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Start Over Author "Régis Hallez" Journal applied and environmental microbiology
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1. Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus

Author

Jean-Jacques Letesson, Régis Hallez, Jean Vandenhaute, and Xavier De Bolle

Subjects
Ecology, biology, Proteome, Genetic Vectors, Brucella abortus, Genetics and Molecular Biology, Gateway (computer program), Computational biology, Origin of replication, biology.organism_classification, Applied Microbiology and Biotechnology, Virology, Epitope, Min System, Vector (molecular biology), Transformation, Bacterial, Food Science, Biotechnology, Brucella melitensis
Abstract

Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus .

Published
2006

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