1. Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp
- Author
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Jelena, Lozo, Nemanja, Mirkovic, Paula M, O'Connor, Milka, Malesevic, Marija, Miljkovic, Natalija, Polovic, Branko, Jovcic, Paul D, Cotter, and Milan, Kojic
- Subjects
Staphylococcus aureus ,Lacticaseibacillus rhamnosus ,food and beverages ,Listeria monocytogenes ,Anti-Bacterial Agents ,Milk ,Bacteriocins ,Cheese ,Operon ,Food Microbiology ,bacteria ,Animals ,Cattle ,Chromatography, High Pressure Liquid ,Lactobacillus plantarum ,Plasmids - Abstract
Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4 produces a novel bacteriocin, lactolisterin BU, with strong antimicrobial activity against many species of Gram-positive bacteria, including important food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU was extracted from the cell surface of BGBU1-4 by 2-propanol and purified to homogeneity by C18 solid-phase extraction and reversed-phase high-performance liquid chromatography. The molecular mass of the purified lactolisterin BU was 5,160.94 Da, and an internal fragment, AVSWAWQH, as determined by N-terminal sequencing, showed low-level similarity to existing antimicrobial peptides. Curing and transformation experiments revealed the presence of a corresponding bacteriocin operon on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4. Analysis of the bacteriocin operon revealed a leaderless bacteriocin of 43 amino acids that exhibited similarity to bacteriocin BHT-B (63%) from Streptococcus ratti, a bacteriocin with analogy to aureocin A.
- Published
- 2017