1. Stress Resistance Development and Genome-Wide Transcriptional Response of Escherichia coli O157:H7 Adapted to Sublethal Thymol, Carvacrol, and trans -Cinnamaldehyde
- Author
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Zi Jing Seng, Liang Yang, Hyun-Gyun Yuk, Wenqian Yuan, Gurjeet S. Kohli, Müller, Volker, School of Biological Sciences, and Singapore Centre for Environmental Life Sciences Engineering
- Subjects
0301 basic medicine ,030106 microbiology ,H7 [E. coli O157] ,Virulence ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Microbiology ,Transcriptome ,03 medical and health sciences ,chemistry.chemical_compound ,medicine ,Carvacrol ,Thymol ,Escherichia coli ,RNA Sequencing ,Ecology ,biology ,Biofilm ,biology.organism_classification ,Science::Biological sciences [DRNTU] ,030104 developmental biology ,chemistry ,Efflux ,Bacteria ,Food Science ,Biotechnology - Abstract
Thymol, carvacrol, and trans-cinnamaldehyde are essential oil (EO) compounds with broad-spectrum antimicrobial activities against foodborne pathogens, including Escherichia coli O157:H7. However, little is known regarding direct resistance and cross-resistance development in E. coli O157:H7 after adaptation to sublethal levels of these compounds, and information is scarce on microbial adaptive responses at a molecular level. The present study demonstrated that E. coli O157:H7 was able to grow in the presence of sublethal thymol (1/2T), carvacrol (1/2C), or trans-cinnamaldehyde (1/2TC), displaying an extended lag phase duration and a lower maximum growth rate. EO-adapted cells developed direct resistance against lethal EO treatments and cross-resistance against heat (58°C) and oxidative (50 mM H2O2) stresses. However, no induction of acid resistance (simulated gastric fluid, pH 1.5) was observed. RNA sequencing revealed a large number (310 to 338) of differentially expressed (adjusted P value [Padj ]
- Published
- 2018
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