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1. Functional analysis of the twin-arginine translocation pathway in Sodalis glossinidius, a bacterial symbiont of the tsetse fly.

Author

De Vooght L, Caljon G, Coosemans M, and Van den Abbeele J

Subjects
Animals, Bacterial Proteins genetics, Enterobacteriaceae classification, Enterobacteriaceae genetics, Escherichia coli Proteins, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Membrane Transport Proteins genetics, Oxidoreductases, N-Demethylating genetics, Oxidoreductases, N-Demethylating metabolism, Bacterial Proteins metabolism, Enterobacteriaceae metabolism, Membrane Transport Proteins metabolism, Symbiosis, Tsetse Flies microbiology
Abstract

This study demonstrates a functional twin-arginine (Tat) translocation pathway present in the tsetse fly symbiont Sodalis glossinidius and its potential to export active heterologous proteins to the periplasm. Functionality was demonstrated using green fluorescent protein (GFP) fused to the Tat signal peptide of Escherichia coli trimethylamine N-oxide reductase (TorA).

Published
2011
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