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Start Over Author "B. Facinelli" Journal applied and environmental microbiology
4 results on '"B. Facinelli"'

1. Genetic basis of tetracycline resistance in food-borne isolates of Listeria innocua

Author

Pietro E. Varaldo, B Facinelli, C Casolari, Marilyn C. Roberts, U. Fabio, and Eleonora Giovanetti

Subjects
DNA, Bacterial, Tetracycline, Listeria, medicine.disease_cause, Applied Microbiology and Biotechnology, Enterococcus faecalis, Microbiology, Listeria monocytogenes, stomatognathic system, Cheese, medicine, polycyclic compounds, Food microbiology, Poultry Products, Ecology, biology, Tetracycline Resistance, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Food Microbiology, Molecular probe, Listeria ivanovii, Bacteria, Food Science, Biotechnology, medicine.drug, Research Article
Abstract

Eleven of 12 tetracycline-resistant Listeria innocua strains, isolated from chicken or turkey frankfurters and mozzarella cheese, were shown to carry DNA sequences which hybridized with the Tet M probe; of these, two strains also hybridized with Tet K. The remaining strain hybridized with the Tet K probe only. The Tet M determinant appeared to be located on the chromosome; in one case, it was transferable by conjugation to recipients Listeria monocytogenes, Listeria ivanovii, and Enterococcus faecalis.

Published
1993

2. Transferable erythromycin resistance in Listeria spp. isolated from food

Author

P E Varaldo, E. Giovanetti, B Facinelli, and Marilyn C. Roberts

Subjects
Listeria, health care facilities, manpower, and services, Erythromycin, medicine.disease_cause, Applied Microbiology and Biotechnology, Enterococcus faecalis, Microbiology, Plasmid, Listeria monocytogenes, health services administration, medicine, Food microbiology, health care economics and organizations, Genetics, Ecology, biology, Genetic transfer, Drug Resistance, Microbial, Methyltransferases, Chromosomes, Bacterial, biology.organism_classification, Genes, Bacterial, Conjugation, Genetic, Food Microbiology, Listeria ivanovii, Research Article, Food Science, Biotechnology, medicine.drug
Abstract

An erythromycin-resistant (Emr) Listeria innocua and an Emr Listeria monocytogenes isolate both carried ermC genes, which code for rRNA methylases. The ermC genes were transferable by conjugation to recipient L. monocytogenes, Listeria ivanovii, and Enterococcus faecalis but did not appear to be associated with conjugative plasmids.

Published
1996
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3. VanA-type enterococci from humans, animals, and food: species distribution, population structure, Tn1546 typing and location, and virulence determinants.

Author

Biavasco F, Foglia G, Paoletti C, Zandri G, Magi G, Guaglianone E, Sundsfjord A, Pruzzo C, Donelli G, and Facinelli B

Subjects
Animals, Bacterial Typing Techniques, Carboxy-Lyases genetics, Chromosomes, Bacterial genetics, DNA Fingerprinting, DNA Transposable Elements genetics, DNA, Bacterial genetics, DNA, Bacterial metabolism, Enterococcus drug effects, Enterococcus isolation & purification, Enterococcus faecalis classification, Enterococcus faecalis drug effects, Enterococcus faecalis genetics, Enterococcus faecalis isolation & purification, Enterococcus faecium classification, Enterococcus faecium drug effects, Enterococcus faecium genetics, Enterococcus faecium isolation & purification, Feces microbiology, Gram-Positive Bacterial Infections epidemiology, Gram-Positive Bacterial Infections transmission, Humans, Meat microbiology, Molecular Epidemiology, Plasmids genetics, Point Mutation, Polymorphism, Restriction Fragment Length, Poultry, Swine, Virulence Factors genetics, Bacterial Proteins genetics, Carbon-Oxygen Ligases genetics, Enterococcus classification, Enterococcus genetics, Food Microbiology, Gram-Positive Bacterial Infections microbiology, Gram-Positive Bacterial Infections veterinary, Vancomycin Resistance genetics
Abstract

VanA-type human (n=69), animal (n=49), and food (n=36) glycopeptide-resistant enterococci (GRE) from different geographic areas were investigated to study their possible reservoirs and transmission routes. Pulsed-field gel electrophoresis (PFGE) revealed two small genetically related clusters, M39 (n=4) and M49 (n=13), representing Enterococcus faecium isolates from animal and human feces and from clinical and fecal human samples. Multilocus sequence typing showed that both belonged to the epidemic lineage of CC17. purK allele analysis of 28 selected isolates revealed that type 1 was prevalent in human strains (8/11) and types 6 and 3 (14/15) were prevalent in poultry (animals and meat). One hundred and five of the 154 VanA GRE isolates, encompassing different species, origins, and PFGE types, were examined for Tn1546 type and location (plasmid or chromosome) and the incidence of virulence determinants. Hybridization of S1- and I-CeuI-digested total DNA revealed a plasmid location in 98% of the isolates. Human intestinal and animal E. faecium isolates bore large (>150 kb) vanA plasmids. Results of PCR-restriction fragment length polymorphism and sequencing showed the presence of prototype Tn1546 in 80% of strains and the G-to-T mutation at position 8234 in three human intestinal and two pork E. faecium isolates. There were no significant associations (P>0.5) between Tn1546 type and GRE source or enterococcal species. Virulence determinants were detected in all reservoirs but were significantly more frequent (P<0.02) among clinical strains. Multiple determinants were found in clinical and meat Enterococcus faecalis isolates. The presence of indistinguishable vanA elements (mostly plasmid borne) and virulence determinants in different species and PFGE-diverse populations in the presence of host-specific purK housekeeping genes suggested that all GRE might be potential reservoirs of resistance determinants and virulence traits transferable to human-adapted clusters.

Published
2007
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4. Genetic basis of tetracycline resistance in food-borne isolates of Listeria innocua.

Author

Facinelli B, Roberts MC, Giovanetti E, Casolari C, Fabio U, and Varaldo PE

Subjects
Cheese microbiology, Listeria classification, Listeria isolation & purification, Poultry Products microbiology, DNA, Bacterial genetics, Food Microbiology, Listeria genetics, Tetracycline Resistance genetics
Abstract

Eleven of 12 tetracycline-resistant Listeria innocua strains, isolated from chicken or turkey frankfurters and mozzarella cheese, were shown to carry DNA sequences which hybridized with the Tet M probe; of these, two strains also hybridized with Tet K. The remaining strain hybridized with the Tet K probe only. The Tet M determinant appeared to be located on the chromosome; in one case, it was transferable by conjugation to recipients Listeria monocytogenes, Listeria ivanovii, and Enterococcus faecalis.

Published
1993
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