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Start Over Author "Manoil, Colin" Journal applied & environmental microbiology Publisher american society for microbiology

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1 results on '"Manoil, Colin"'

1. Exploiting a Natural Auxotrophy for Genetic Selection.

Author

Ramage, Elizabeth, Gallagher, Larry, and Manoil, Colin

Subjects
*HISTIDINE, *AUXOTROPHY, *DEHYDROGENASES, *BIOMARKERS, *FRANCISELLA, *BACTERIAL growth, *ESCHERICHIA coli
Abstract

We exploited the natural histidine auxotrophy of FrawciseHa species to develop hisD (encodes histidinol dehydrogenase) as a positive selection marker. A shuttle plasmid (pBR103) carrying Escherichia coli hisD and designed for cloning of PCR fragments replicated in both attenuated and highly virulent Francisella strains. During this work, we formulated a simplified defined growth medium for Francisella novicida. [ABSTRACT FROM AUTHOR]

Published
2012
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