1. Development of microsatellite loci of pod mahogany, Afzelia quanzensis (Fabaceae), by Illumina shotgun sequencing, and cross-amplification in A. africana
- Author
-
Percy Jinga, Mary V. Ashley, and Jason Palagi
- Subjects
0106 biological sciences ,0301 basic medicine ,Afzelia africana ,microsatellite ,PAL_FINDER ,Plant Science ,010603 evolutionary biology ,01 natural sciences ,03 medical and health sciences ,Illumina ,Polymorphism (computer science) ,Genotyping ,Ecology, Evolution, Behavior and Systematics ,Genetics ,Afzelia quanzensis ,Genetic diversity ,biology ,Shotgun sequencing ,food and beverages ,Fabaceae ,biology.organism_classification ,Primer Note ,030104 developmental biology ,Microsatellite - Abstract
Premise of the study: Microsatellite loci were developed for Afzelia quanzensis (Fabaceae) as a first step toward investigating genetic diversity and population structure of the species in its native range. Methods and Results: Illumina shotgun sequencing was used to generate raw sequence reads, which were searched for potential microsatellite loci. A total of 70 potential microsatellite loci were tested for amplification and polymorphism, and 39 successfully amplified. Of the 39 loci that amplified, 12 were polymorphic while 27 were monomorphic. The 12 polymorphic loci were cross-amplified in A. africana, and eight successfully amplified. Conclusions: The 12 polymorphic microsatellite loci can be used for genetic studies of A. quanzensis, which can help determine its conservation status. Eight loci can also be used for genotyping in A. africana.
- Published
- 2016