1. Evaluation of minority populations of HIV type-1 with K103N and M184V drug resistance mutations among children in Argentina
- Author
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Graciela Barboni, Jorge Quarleri, Silvia González Ayala, Mariel García, Moira Vignoles, Horacio Salomón, and María Rosa Agosti
- Subjects
Male ,Adolescent ,Anti-HIV Agents ,Population ,Argentina ,HIV Infections ,Kaplan-Meier Estimate ,Drug resistance ,Polymerase Chain Reaction ,Drug Administration Schedule ,Acquired immunodeficiency syndrome (AIDS) ,Antiretroviral Therapy, Highly Active ,Drug Resistance, Viral ,medicine ,Humans ,Pharmacology (medical) ,Nevirapine ,Selection, Genetic ,Child ,education ,Sida ,Pharmacology ,education.field_of_study ,biology ,business.industry ,Transmission (medicine) ,Infant ,virus diseases ,Sequence Analysis, DNA ,medicine.disease ,Resistance mutation ,biology.organism_classification ,Genes, pol ,Virology ,Infectious Diseases ,Lamivudine ,Child, Preschool ,Mutation ,Lentivirus ,HIV-1 ,Reverse Transcriptase Inhibitors ,Female ,Viral disease ,business - Abstract
Background The aim of this study was to describe the frequency of minority populations of viruses carrying mutations K103N and M184V in drug-naive HIV type-1 (HIV-1)-infected children, and to further evaluate their effect on the selection of drug-resistant viruses within highly active antiretroviral therapy (HAART). Methods Newly diagnosed vertically HIV-1-infected children were evaluated. The HIV-1 pol gene was sequenced for subtyping and antiretroviral drug resistance analysis. Standard genotypic sequencing and sequence-selective real-time PCR (SPCR) to quantify minority viral populations were used. Results From December 2004 to July 2006, we included 35 children who were studied at baseline and during their first HAART regimen (follow-up median time 29.4 months). Of them, 82.9% were infected with intersubtype B/F recombinant variants. At baseline, all children had a drug-susceptible viral population that was studied by bulk sequencing. SPCR showed that 4 children had between 2–10% of M184V, 11 had 20% in less time than those with 0.1–0.6% or without minority populations ( P=0.01). Conclusions It was shown that having 2–10% of M184V at baseline enhanced its selection in high percentages in a short time after HAART initiation. Further research regarding the presence of minority quasispecies before initiation of HAART in large paediatric populations should be undertaken to evaluate their clinical effect during HAART.
- Published
- 2009