Search

Your search keyword '"The University of Texas Medical Branch ( UTMB )"' showing total 3 results
Start Over Author "The University of Texas Medical Branch ( UTMB )" Journal antiviral research
3 results on '"The University of Texas Medical Branch ( UTMB )"'

1. AT-752 targets multiple sites and activities on the Dengue virus replication enzyme NS5

Author

Mikael Feracci, Cécilia Eydoux, Véronique Fattorini, Lea Lo Bello, Pierre Gauffre, Barbara Selisko, Priscila Sutto-Ortiz, Ashleigh Shannon, Hongjie Xia, Pei-Yong Shi, Mathieu Noel, Françoise Debart, Jean-Jacques Vasseur, Steve Good, Kai Lin, Adel Moussa, Jean-Pierre Sommadossi, Aurélie Chazot, Karine Alvarez, Jean-Claude Guillemot, Etienne Decroly, François Ferron, Bruno Canard, Architecture et fonction des macromolécules biologiques (AFMB), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), The University of Texas Medical Branch (UTMB), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Université de Montpellier (UM), and European Virus Bioinformatics Center [Jena]

Subjects
STRUCTURAL BASIS, Pharmacology, RNAPOLYMERASE, Virology, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, 2'-OPROTEIN MOTIF, [CHIM.THER]Chemical Sciences/Medicinal Chemistry
Abstract

International audience; AT-752 is a guanosine analogue prodrug active against dengue virus (DENV). In infected cells, it is metabolized into 2 '-methyl-2 '-fluoro guanosine 5 '-triphosphate (AT-9010) which inhibits RNA synthesis in acting as a RNA chain terminator. Here we show that AT-9010 has several modes of action on DENV full-length NS5. AT-9010 does not inhibit the primer pppApG synthesis step significantly. However, AT-9010 targets two NS5-associated enzyme activities, the RNA 2 '-O-MTase and the RNA-dependent RNA polymerase (RdRp) at its RNA elongation step. Crystal structure and RNA methyltransferase (MTase) activities of the DENV 2 MTase domain in complex with AT-9010 at 1.97 angstrom resolution shows the latter bound to the GTP/RNA-cap binding site, accounting for the observed inhibition of 2 '-O but not N7-methylation activity. AT-9010 is discriminated-10 to 14-fold against GTP at the NS5 active site of all four DENV1-4 NS5 RdRps, arguing for significant inhibi-tion through viral RNA synthesis termination. In Huh-7 cells, DENV1-4 are equally sensitive to AT-281, the free base of AT-752 (EC50 approximate to 0.50 mu M), suggesting broad spectrum antiviral properties of AT-752 against flaviviruses.

Published
2023
Full Text
View/download PDF

2. Zika in the Americas, year 2: What have we learned? What gaps remain? A report from the Global Virus Network

Author

Matthew T. Aliota, Nikos Vasilakis, Edward McSweegan, Leda Bassit, Michael J. Ricciardi, Thomas C. Friedrich, Guilherme S. Ribeiro, Natalia Mercer, Geraldine Schott-Lerner, George R. Saade, Thaddeus G. Golos, Shelton S. Bradrick, Diane E. Griffin, Mariano A. Garcia-Blanco, Lisa F. P. Ng, Pei Yong Shi, Diogo M. Magnani, Bryan Cox, Christina Gavegnano, Marc Lecuit, Chao Shan, Caroline Marrs, Andrew D. Haddow, David I. Watkins, Jorge E. Osorio, Scott C. Weaver, Raymond F. Schinazi, David H. O’Connor, Esper G. Kallas, Shannan L. Rossi, Uriel Kitron, University of Wisconsin-Madison, Emory University [Atlanta, GA], The University of Texas Medical Branch (UTMB), Global Virus Network, University of South Florida [Tampa] (USF), Johns Hopkins Bloomberg School of Public Health [Baltimore], Johns Hopkins University (JHU), U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID), Universidade de São Paulo = University of São Paulo (USP), Biologie des Infections - Biology of Infection, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), University of Miami Leonard M. Miller School of Medicine (UMMSM), Agency for science, technology and research [Singapore] (A*STAR), Universidade Federal da Bahia (UFBA), Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP), Work from the authors’ laboratories was funded by in part by U.S. National Institutes of Health grants: R01NS087539-S1 (to DEG), R21AI129607 (to RFS), and R24AI120942, R01AI121452 (to SCW) R01 AI107157-01A1 (to TGG), R01AI116382-01A1S1 (to DHO), U01AI115577 (to NV) and P51 OD011106 (to the WNPRC)., U.S. Army Medical Research Institute of Infectious Diseases, University of São Paulo (USP), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut des Maladies Génétiques Imagine [Paris], Fundação Oswaldo Cruz (FIOCRUZ), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)

Subjects
0301 basic medicine, Gerontology, medicine.medical_specialty, Asia, Sexual transmission, MALFORMAÇÕES, [SDV]Life Sciences [q-bio], Disease, Antiviral therapy, Nervous System Malformations, Arbovirus, Article, Zika virus, 03 medical and health sciences, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Virology, Drug Discovery, Pandemic, Disease Transmission, Infectious, medicine, Animals, Maternal-fetal transmission, Pharmacology, Vaccines, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, biology, Diagnostic Tests, Routine, Zika Virus Infection, business.industry, Transmission (medicine), Public health, Outbreak, medicine.disease, biology.organism_classification, Infectious Disease Transmission, Vertical, 3. Good health, Congenital manifestations, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, 030104 developmental biology, Family medicine, Africa, Communicable Disease Control, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Americas, business, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience; In response to the outbreak of Zika virus (ZIKV) infection in the Western Hemisphere and the recognition of a causal association with fetal malformations, the Global Virus Network (GVN) assembled an international taskforce of virologists to promote basic research, recommend public health measures and encourage the rapid development of vaccines, antiviral therapies and new diagnostic tests. In this article, taskforce members and other experts review what has been learned about ZIKV-induced disease in humans, its modes of transmission and the cause and nature of associated congenital manifestations. After describing the make-up of the taskforce, we summarize the emergence of ZIKV in the Americas, Africa and Asia, its spread by mosquitoes, and current control measures. We then review the spectrum of primary ZIKV-induced disease in adults and children, sites of persistent infection and sexual transmission, then examine what has been learned about maternal-fetal transmission and the congenital Zika syndrome, including knowledge obtained from studies in laboratory animals. Subsequent sections focus on vaccine development, antiviral therapeutics and new diagnostic tests. After reviewing current understanding of the mechanisms of emergence of Zika virus, we consider the likely future of the pandemic.

Published
2017
Full Text
View/download PDF

3. GloPID-R report on chikungunya, o'nyong-nyong and Mayaro virus, part 2: Epidemiological distribution of o'nyong-nyong virus

Author

M. G. Rosa-Freitas, Sébastien Boyer, A. J. Rodriguez-Morales, Jan Felix Drexler, Thomas Jaenisch, Patrícia Brasil, Johan Neyts, François Simon, Anna-Bella Failloux, de Lamballerie X, Lisa F. P. Ng, Michael A. Drebot, Pierre Gallian, Guilherme S. Ribeiro, Maria Rios, Amadou A. Sall, Chantal Reusken, Marc Lecuit, Scott C. Weaver, Michael P. Busch, Nikos Vasilakis, André Siqueira, A. Vega Rua, Angelle Desiree LaBeaud, Mawlouth Diallo, Michael S. Diamond, Alain Kohl, Ricardo Lourenço-de-Oliveira, Laura Pezzi, Graham Simmons, Unité des Virus Emergents (UVE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Virologie [UNIV Corse-Inserm] (EA7310), Université Pascal Paoli (UPP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Stanford School of Medicine [Stanford], Stanford Medicine, Stanford University-Stanford University, National Institute for Public Health and the Environment [Bilthoven] (RIVM), Erasmus University Medical Center [Rotterdam] (Erasmus MC), Charité - UniversitätsMedizin = Charité - University Hospital [Berlin], Humboldt University Of Berlin, Berlin Institute of Health (BIH), The University of Texas Medical Branch (UTMB), Institut Pasteur de Dakar, Réseau International des Instituts Pasteur (RIIP), Hôpital d'Instruction des Armées Laveran, Service de Santé des Armées, Heidelberg University Hospital [Heidelberg], Etablissement Français du Sang - Alpes-Méditerranée (EFS - Alpes-Méditerranée), Etablissement Français du Sang, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), Institut Pasteur du Cambodge, Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ), University of California [San Francisco] (UC San Francisco), University of California (UC), Washington University in Saint Louis (WUSTL), Public Health Agency of Canada, MRC - University of Glasgow Centre for Virus Research, Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Biologie des Infections - Biology of Infection, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), Agency for science, technology and research [Singapore] (A*STAR), Universidade Federal da Bahia (UFBA), U.S. Food and Drug Administration (FDA), Universidad Tecnológica de Pereira [Colombie] (UTP), Institut Pasteur de la Guadeloupe, Publication fees were covered by the Global Research Collaboration for Infectious Disease Preparedness (GloPID-R)., Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pascal Paoli (UPP), Humboldt-Universität zu Berlin, Institut Pasteur [Paris], Fundação Oswaldo Cruz (FIOCRUZ), University of California [San Francisco] (UCSF), University of California, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Virology, and DEMESLAY GOUGAM, MARIE

Subjects
0301 basic medicine, GloPID-R chikungunya, o'nyong-nyong and Mayaro virus Working Group, Genes, Viral, Iron, 030106 microbiology, Prevalence, Alphavirus, medicine.disease_cause, Disease Outbreaks, 03 medical and health sciences, Seroepidemiologic Studies, Virology, Environmental health, medicine, Seroprevalence, Animals, Humans, O'nyong-nyong Virus, Serologic Tests, Chikungunya, Phylogeny, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Pharmacology, Molecular epidemiology, Alphavirus Infections, Outbreak, 3. Good health, 030104 developmental biology, Geography, Infectious disease (medical specialty), [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Africa, Chikungunya Fever, Chikungunya virus
Abstract

The GloPID-R (Global Research Collaboration for Infectious Disease Preparedness) chikungunya (CHIKV), o'nyong-nyong (ONNV) and Mayaro virus (MAYV) Working Group has been established to identify gaps of knowledge about the natural history, epidemiology and medical management of infection by these viruses, and to provide adapted recommendations for future investigations. Here, we present a report dedicated to ONNV epidemiological distribution. Two large-scale ONNV outbreaks have been identified in Africa in the last 60 years, interspersed with sporadic serosurveys and case reports of returning travelers. The assessment of the real scale of ONNV circulation in Africa remains a difficult task and surveillance studies are necessary to fill this gap. The identification of ONNV etiology is made complicated by the absence of multiplex tools in co-circulation areas and that of reference standards, as well as the high cross-reactivity with related pathogens observed in serological tests, in particular with CHIKV. This is a specific obstacle for seroprevalence studies, that necessitate an improvement of serological tools to provide robust results. The scarcity of existent genetic data currently limits molecular epidemiology studies. ONNV epidemiology would also benefit from reinforced entomological and environmental surveillance. Finally, the natural history of the disease deserves to be further investigated, with a specific attention paid to long-term complications. Considering our incomplete knowledge on ONNV distribution, GloPID-R CHIKV, ONNV and MAYV experts recommend that a major effort should be done to fill existing gaps. ispartof: ANTIVIRAL RESEARCH vol:172 ispartof: location:Netherlands status: published

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results