1. The protective immune response against Pseudorabies virus induced by DNA vaccination is impaired if the plasmid harbors a functional Porcine circovirus type 2 rep and origin of replication.
- Author
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Faurez F, Grasland B, Béven V, Cariolet R, Keranflec'h A, Henry A, Jestin A, and Dory D
- Subjects
- Animals, Antibodies, Viral immunology, DNA Replication, Female, Genes, Viral, Herpesvirus 1, Suid immunology, Interferon-gamma immunology, Plasmids genetics, Pseudorabies immunology, Pseudorabies virology, Specific Pathogen-Free Organisms, Swine immunology, Swine virology, Swine Diseases immunology, Swine Diseases prevention & control, Swine Diseases virology, Vaccination, Vaccines, DNA genetics, Viral Vaccines genetics, Circovirus genetics, Herpesvirus 1, Suid pathogenicity, Pseudorabies prevention & control, Replication Origin, Vaccines, DNA immunology, Viral Vaccines immunology
- Abstract
A plasmid rendered replicative in mammalian cells by inserting the Porcine circovirus 2 (PCV2) origin of replication and replicase gene (Ori-rep) has been previously constructed. The aim of the present study was to evaluate if the replication capacity of this plasmid could be advantageously used to improve the protective immunity induced by DNA vaccination. In this case we used the porcine Pseudorabies virus (PrV) DNA vaccination model. The replicative capacity of the DNA vaccine did not improve the protective immunity against PrV in pigs, but on the contrary the presence of the PCV2 Ori-rep sequence was harmful in the induction of this immunity compared to an equivalent but non-replicative DNA vaccine. In addition, the distribution and the persistence of the replicative and non-replicative plasmids inside the body were the same. This is the first study showing an in vivo deleterious effect of the replicative active PCV2 Ori-rep on the natural and specific protection against PrV infection., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2012
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