1. Knockdown of Importin 7 Inhibits Lung Tumorigenesis in K-rasLA1 Lung Cancer Mice
- Author
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Seong-Ho Hong, Somin Lee, Sangbum Kim, Hu-Lin Jiang, Myung-Haing Cho, Sang Wha Kim, and Ah Young Lee
- Subjects
0301 basic medicine ,Cancer Research ,Gene knockdown ,animal structures ,Chemistry ,General Medicine ,respiratory system ,Gene delivery ,medicine.disease ,medicine.disease_cause ,03 medical and health sciences ,030104 developmental biology ,Oncology ,In vivo ,Apoptosis ,Cancer research ,medicine ,Adenocarcinoma ,Lung cancer ,Carcinogenesis ,Protein kinase B - Abstract
Background/Aim: Lung cancer shows the highest estimated deaths in both males and females in the Unites States. Importin 7 is overexpressed in lung adenocarcinoma tissues. In this study, we aimed to demonstrate the anticancer effect of importin 7 down-regulation, especially in lung cancer. Materials and Methods: Glycerol propoxylate triacrylate spermine (GPT-SPE) is a biocompatible carrier used for aerosol gene delivery. Repeated aerosol delivery of GPT-SPE/shImportin 7 complexes was performed to 10-week-old male K-rasLA1mice (a murine lung cancer model) twice a week for 4 weeks (8 times) in a nose-only exposure chamber. Results: Aerosol delivery of GPT-SPE/shImportin 7 inhibits lung cancer in K-rasLA1mice compared to control and scramble control groups. Moreover, importin 7-down-regulated stable cell-line demonstrates suppression of proliferation through Akt inhibition and apoptosis. Conclusion: Down-regulation of importin 7 significantly suppresses lung cancer in vitro and in vivo.
- Published
- 2017