1. Exploring the Role of Enzalutamide in Combination with Radiation Therapy: An
- Author
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Luca, Triggiani, Antonella, Colosini, Michela, Buglione, Nadia, Pasinetti, Flavia, Orizio, Lilia, Bardoscia, Paolo, Borghetti, Marta, Maddalo, Luigi, Spiazzi, Stefano Maria, Magrini, and Roberto, Bresciani
- Subjects
Male ,Radiation-Sensitizing Agents ,Cell Survival ,Prostatic Neoplasms ,Antineoplastic Agents ,Dose-Response Relationship, Radiation ,Histones ,Drug Resistance, Neoplasm ,Cell Line, Tumor ,Benzamides ,Nitriles ,Phenylthiohydantoin ,Humans ,Cell Proliferation - Abstract
Androgen receptor plays a key role in prostate cancer development and is a causative agent of its radio-resistance. The present study investigated the potential radio-sensitizing effect of enzalutamide, a second-generation anti-androgen, in human prostate cancer cells.The radio-sensitizing effect of enzalutamide was assessed in the androgen-dependent LNCaP cells and the androgen-independent PC3 cells by clonogenic assay and γ-H2AX assay.Enzalutamide-treated LNCaP cells showed a significant decrease of cell survival at all radiation doses tested. An increased number of γ-H2AX-positive nuclei was observed, suggesting a possible impairment of the DNA repair machinery. Conversely, enzalutamide did not exhibit a significant radio-sensitizing effect on PC3 cells.The combination of enzalutamide with ionizing radiation significantly improves radio-sensitivity of hormone-dependent LNCaP cells. Translated in the clinical practice, our results may help to find additional strategies to improve effectiveness of radiotherapy.
- Published
- 2018