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Your search keyword '"H. Poppleton"' showing total 2 results
Start Over Author "H. Poppleton" Journal anticancer research
2 results on '"H. Poppleton"'

1. Maturation of tumor vasculature by interferon-beta disrupts the vascular niche of glioma stem cells

Author

R F, Williams, T L, Sims, L, Tracey, A L, Myers, C Y C, Ng, H, Poppleton, A C, Nathwani, and A M, Davidoff

Subjects
Male, Brain Neoplasms, Endothelial Cells, Fluorescent Antibody Technique, Antineoplastic Agents, Cell Communication, Glioma, Interferon-beta, Mice, SCID, Xenograft Model Antitumor Assays, Coculture Techniques, Mice, Neoplastic Stem Cells, Animals, Humans, Pericytes
Abstract

The vascular niche necessary for cancer stem cell maintenance is a potential target for cancer therapy.Human glioma xenografts were treated with IFN-β delivered systemically via a liver-targeted, adeno-associated viral vector. The vascular niche was examined with immunofluorescence for glioma stem cells, endothelial cells, and perivascular cells.Although IFN-β was not directly toxic to glioma stem cells in vitro, IFN-β decreased tumor size and the number of stem cells recovered in both heterotopic and orthotopic models. Treatment with IFN-β increased perivascular cells investing the tumor vasculature (6-fold) distancing stem cells from endothelial cells. Additionally, vascular smooth muscle cells co-cultured between stem cells and endothelial cells decreased stem cell recovery.Continuous delivery of IFN-β decreased the number of stem cells in glioma xenografts by disrupting the vascular niche through an increase in perivascular cells, which created a barrier between the glioma stem cells and the endothelial cells.

Published
2010

2. Maturation of tumor vasculature by interferon-beta disrupts the vascular niche of glioma stem cells.

Author

Williams RF, Sims TL, Tracey L, Myers AL, Ng CY, Poppleton H, Nathwani AC, and Davidoff AM

Subjects
Animals, Brain Neoplasms drug therapy, Brain Neoplasms metabolism, Cell Communication drug effects, Coculture Techniques, Endothelial Cells drug effects, Fluorescent Antibody Technique, Glioma drug therapy, Glioma metabolism, Humans, Male, Mice, Mice, SCID, Pericytes drug effects, Xenograft Model Antitumor Assays, Antineoplastic Agents pharmacology, Brain Neoplasms blood supply, Glioma blood supply, Interferon-beta pharmacology, Neoplastic Stem Cells drug effects
Abstract

Background: The vascular niche necessary for cancer stem cell maintenance is a potential target for cancer therapy., Materials and Methods: Human glioma xenografts were treated with IFN-β delivered systemically via a liver-targeted, adeno-associated viral vector. The vascular niche was examined with immunofluorescence for glioma stem cells, endothelial cells, and perivascular cells., Results: Although IFN-β was not directly toxic to glioma stem cells in vitro, IFN-β decreased tumor size and the number of stem cells recovered in both heterotopic and orthotopic models. Treatment with IFN-β increased perivascular cells investing the tumor vasculature (6-fold) distancing stem cells from endothelial cells. Additionally, vascular smooth muscle cells co-cultured between stem cells and endothelial cells decreased stem cell recovery., Conclusion: Continuous delivery of IFN-β decreased the number of stem cells in glioma xenografts by disrupting the vascular niche through an increase in perivascular cells, which created a barrier between the glioma stem cells and the endothelial cells.

Published
2010

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