1. Chemopreventive potential of black cohosh on breast cancer in Sprague-Dawley rats
- Author
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Linda Saxe, Einbond, Morando, Soffritti, Davide, Degli Esposti, Eva, Tibaldi, Michelina, Lauriola, Luciano, Bua, Kan, He, Giannicola, Genovese, Tao, Su, Lesley, Huggins, Xiaomei, Wang, Marc, Roller, and Hsan-Au, Wu
- Subjects
Immunoenzyme Techniques ,Rats, Sprague-Dawley ,Survival Rate ,Cimicifuga ,Fibroadenoma ,Plant Extracts ,Animals ,Female ,Mammary Neoplasms, Animal ,Adenocarcinoma ,Cell Proliferation ,Phytotherapy ,Rats - Abstract
This study examines the chemopreventive potential and action of the herb black cohosh on Sprague-Dawley rats.Female Sprague-Dawley rats were treated with an extract of black cohosh enriched in triterpene glycosides (27%) at 35.7 (Group I), 7.14 (Group II), 0.714 (Group III) or 0 mg/kg b.w. for 40 weeks starting from 56 weeks of age and the incidence of benign and malignant mammary tumors was determined at the end of observation.Among female rats treated at 35.7 and 7.14 mg/kg b.w. there was a dose-related reduction (p0.05) of the incidence of mammary adenocarcinomas when compared to the treatment of 0.714 mg/kg b.w., with a protection index (calculated relative to the group III; PI=[total tumours × 100 animals of group III] - [total tumours × 100 animals of the group I (or group II)]/ [total tumours of group III] × 100) for mammary adenocarcinomas of 87.5 and 48.8%, respectively. Black cohosh reduced Ki-67 and cyclin D1 protein expression in fibroadenomas, by immunohistochemistry.Our results suggest that black cohosh may have chemopreventive potential for mammary cancer.
- Published
- 2012