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Your search keyword '"Manon E. Wildenberg"' showing total 2 results
Start Over Author "Manon E. Wildenberg" Journal annals of the rheumatic diseases
2 results on '"Manon E. Wildenberg"'

1. Prevalence of interferon type I signature in CD14 monocytes of patients with Sjögren's syndrome and association with disease activity and BAFF gene expression

Author

Virgil A. S. H. Dalm, Manon E Wildenberg, Zana Brkic, Marjan A. Versnel, Paul L A van Daele, Hemmo A. Drexhage, Cornelia G van Helden-Meeuwsen, Joop P. van de Merwe, Naomi I Maria, Wouter Beumer, Immunology, Internal Medicine, AII - Amsterdam institute for Infection and Immunity, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, and Gastroenterology and Hepatology

Subjects
Adult, Male, CD14, Lymphocyte, Immunology, Lipopolysaccharide Receptors, Gene Expression, Real-Time Polymerase Chain Reaction, Monocytes, General Biochemistry, Genetics and Molecular Biology, stomatognathic system, Rheumatology, Interferon, B-Cell Activating Factor, Prevalence, medicine, Humans, Immunology and Allergy, Rheumatoid factor, RNA, Messenger, B-cell activating factor, Aged, business.industry, Monocyte, Autoantibody, Middle Aged, Clinical and Epidemiological Research, Up-Regulation, stomatognathic diseases, Sjogren's Syndrome, medicine.anatomical_structure, Interferon Type I, Female, business, Biomarkers, Interferon type I, medicine.drug
Abstract

Objective To determine the prevalence of upregulation of interferon (IFN) type I inducible genes, the so called ‘IFN type I signature’, in CD14 monocytes in 69 patients with primary Sjögren's syndrome (pSS) and 44 healthy controls (HC) and correlate it with disease manifestations and expression of B cell activating factor (BAFF). Methods Expression of IFI44L, IFI44, IFIT3, LY6E and MX1 was measured using real time quantitative PCR in monocytes. Expression values were used to calculate IFN type I scores for each subject. pSS patients positive for the IFN type I signature (IFN score≥10) and patients negative for the signature (IFN score

Published
2012

2. Increased frequency of CD16+monocytes and the presence of activated dendritic cells in salivary glands in primary Sjogren syndrome

Author

Manon E. Wildenberg, Hemmo A. Drexhage, C.G. van Helden-Meeuwsen, Jojanneke M. C. Welzen-Coppens, Hendrika Bootsma, Marjan A. Versnel, Arjan Vissink, N. van Rooijen, J. P. van de Merwe, Personalized Healthcare Technology (PHT), Translational Immunology Groningen (TRIGR), Tytgat Institute for Liver and Intestinal Research, Molecular cell biology and Immunology, CCA - Immuno-pathogenesis, and Immunology

Subjects
Monocytes, Salivary Glands, Immature Monocyte, Mice, Immunophenotyping, MOUSE MODELS, Mice, Inbred NOD, CD16+ BLOOD MONOCYTES, FC-GAMMA, Immunology and Allergy, SYSTEMIC-LUPUS-ERYTHEMATOSUS, Cells, Cultured, education.field_of_study, Membrane Glycoproteins, Salivary gland, IMMUNE-RESPONSES, Cell Differentiation, hemic and immune systems, Middle Aged, medicine.anatomical_structure, Sjogren's Syndrome, CD14(+)CD16(+) MONOCYTES, Cytokines, TISSUE MACROPHAGES, Adult, Immunology, Population, CD11c, Immunoglobulins, chemical and pharmacologic phenomena, CD16, GPI-Linked Proteins, General Biochemistry, Genetics and Molecular Biology, Rheumatology, SDG 3 - Good Health and Well-being, Antigens, CD, medicine, Animals, Humans, education, Aged, business.industry, Monocyte, Receptors, IgG, Lysosome-Associated Membrane Glycoproteins, Dendritic cell, Dendritic Cells, IN-VITRO, RHEUMATOID-ARTHRITIS, T-CELLS, business
Abstract

Objectives:In the salivary glands of patients with primary Sjögren Syndrome (pSjS) an accumulation of dendritic cells (DCs) is seen, which is thought to play a role in stimulating local inflammation. Aberrancies in subsets of monocytes, generally considered the blood precursors for DCs, may play a role in this accumulation of DCs. This study is aimed at determining the level of mature CD14lowCD16+ monocytes in pSjS and their contribution to the accumulation of DCs in pSjS.Methods:Levels of mature and immature monocytes in patients with pSjS (n = 19) and controls (n = 15) were analysed by flow cytometry. The reverse transmigration system was used for generation of DCs generated from monocyte subsets. The phenotype of DCs in pSjS salivary glands was analysed using immunohistochemistry. In vivo tracking of monocyte subsets was performed in a mouse model.Results:Increased levels of mature CD14lowCD16+ monocytes were found in patients with pSjS (mean (SD) 14.5 (5.5)% vs 11.4 (3.4)%). These cells showed normal expression of chemokine receptor and adhesion molecules. Mature monocytes partly developed into DC-lysosome-associated membrane glycoprotein (LAMP)+ (19.6 (7.5)%) and CD83+ (16 (9)%) DCs, markers also expressed by DCs in pSjS salivary glands. Monocyte tracking in the non-obese diabetic (NOD) mouse showed that the homologue population of mature mouse monocytes migrated to the salivary glands, and preferentially developed into CD11c+ DCs in vivo.Conclusions:Mature monocytes are increased in pSjS and patient and mouse data support a model where this mature monocyte subset migrates to the salivary glands and develops into DCs.

Published
2009

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