1. Rescue of cells from apoptosis by antioxidants occurs downstream from GSH extrusion
- Author
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Andrea Magrini, Milena Denicola, Lina Ghibelli, Maria Rosa Ciriolo, Antonio Bergamaschi, Maria D'alessio, Francesca Liuzzi, C. Fanelli, and Claudia Cerella
- Subjects
antioxidant ,cell protection ,Reducing agent ,Lethrinidae ,Cell Survival ,Radical ,2 oxobutyric acid ,Apoptosis ,General Biochemistry, Genetics and Molecular Biology ,2 oxoglutaric acid ,Antioxidants ,chemistry.chemical_compound ,History and Philosophy of Science ,Downstream (manufacturing) ,Settore MED/44 - Medicina del Lavoro ,oxidative stress ,Humans ,controlled study ,human ,Anaerobiosis ,2 oxoisovaleric acid ,dithiothreitol ,glutathione ,scavenger ,thiol ,apoptosis ,cell culture ,cell damage ,conference paper ,downstream processing ,human cell ,protein expression ,Cell Hypoxia ,Glutathione ,Puromycin ,U937 Cells ,chemistry.chemical_classification ,U937 cell ,General Neuroscience ,Cell biology ,chemistry ,Biochemistry ,Thiol ,Extrusion - Abstract
Antioxidants-that is, scavengers of free radicals and anaerobic conditions (5%CO(2), 95% N(2))-protect monocytic U937 cells from damage-induced apoptosis. Antioxidants rescue the cells acting on the apoptotic pathway at a step downstream from gluthatione extrusion. Reducing agents, such as DTT, also reduce stress-induced apoptosis. Thus, apoptotic GSH extrusion triggers the downstream events of apoptosis by leaving cells unprotected against thiol\s oxidation and radical production.
- Published
- 2004