1. Assay for urinary desmosines in a healthy pre-pubertal population using an improved extraction technique
- Author
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Siobhain Brennan, G Neil Kent, Peter D. Sly, Kaye Winfield, and Samantha Gard
- Subjects
Adolescent ,Urinary system ,Clinical Biochemistry ,Population ,Physiology ,Reference range ,Urine ,Desmosine ,Excretion ,chemistry.chemical_compound ,Humans ,Isodesmosine ,education ,Child ,Chromatography, High Pressure Liquid ,education.field_of_study ,Chromatography ,biology ,Hydrolysis ,Infant, Newborn ,Infant ,General Medicine ,chemistry ,Child, Preschool ,biology.protein ,Female ,Elastin - Abstract
Background: Current evidence indicates that increased desmosine excretion reflects the active inflammatory status of some connective tissue diseases. Our goal was to establish a reliable method of detection and to investigate the normal distribution of urinary desmosine excretion in a healthy pre-pubertal population. Method: Urine was collected from healthy volunteers aged four weeks to 12 years old. We modified a published high-performance liquid chromatography (HPLC) method by (a) increasing hydrolysis time and temperature and (b) increasing cellulose column size. Results: Our modified method had small inter- and intra-assay variability, with coefficients of variation of 2 = 0.91). There was no significant diurnal or day-to-day variability in total desmosine levels. A reference range for healthy pre-pubertal children aged four weeks to 12 years was established. Conclusion: The modified HPLC method is reliable with low variability. The technique can now be applied as a non-invasive research or diagnostic tool for children with chronic lung disease.
- Published
- 2006